Study of inhaled nanoparticles incorporating synthetic oligodeoxynucleotides to treat lung diseases

掺入合成寡脱氧核苷酸的吸入纳米颗粒治疗肺部疾病的研究

• 批准号: 23790917
• 负责人: SATO Takashi
• 金额: $ 2.75万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790917/
• 关键词: 免疫調節性 DNA; ナノ粒子; 免疫制御

We have developed biodegradable polyketal nano-particles containing immune-modulatory oligodeoxynucleotides (PKN-ODN), that degraded as predicted under physiologic conditions. To evaluate the ability of PKN-ODN to reduce pulmonary inflammation, well established silicosis and elastase-induced emphysema mouse models were used to develop the technology for the endotracheal delivery of PKN-ODN designed for the treatment pulmonary inflammation and the subsequent monitoring of the outcome of such intervention by Flexivent pulmonary function measuring system. In murine emphysema model, single intratracheal instillation of immune-stimulatory ODN could improve the changes in lung function parameters (Resistance, Elastance, Compliance), implying a potential treatment option.

我们已经开发了可生物降解的聚缩酮纳米颗粒含有免疫调节寡脱氧核苷酸（PKN-ODN），降解预测在生理条件下。为了评价PKN-ODN减轻肺部炎症的能力，使用良好建立的矽肺和弹性蛋白酶诱导的肺气肿小鼠模型来开发用于治疗肺部炎症的PKN-ODN的气管内递送技术，并随后通过呼吸机肺功能测量系统监测这种干预的结果。在小鼠肺气肿模型中，单次气管内滴注免疫刺激性ODN可以改善肺功能参数（阻力、弹性、顺应性）的变化，这意味着一种潜在的治疗选择。

项目成果

Serum Heme Oxygenase-1 As Marker Of Lung Function Decline In Patients With Chronic Silicosis

血清血红素氧合酶 1 作为慢性矽肺患者肺功能下降的标志

• 发表时间: 2011
• 作者: Sato T;Miyazawa N;Saito Y;Takahashi R;et al
• 通讯作者: et al

Suppressive oligodenoxynucleotide synergistically enhances the antiproliferative effect of anti-cancer drug in human lung cancer cells

抑制性寡聚脱氧核苷酸协同增强抗癌药物对人肺癌细胞的抗增殖作用

• 发表时间: 2011
• 作者: Takahashi R;Sato T;Miyazawa N;et al.
• 通讯作者: et al.

CpG oligodeoxynucleotide promote wound healing in airway epithelial cell

CpG寡脱氧核苷酸促进气道上皮细胞伤口愈合

• 发表时间: 2011
• 作者: Sato T;Miyazawa N;Klinman D;et al.
• 通讯作者: et al.

Suppressive Oligodeoxynucleotide Synergistically Enhances The Antiproliferative Effect Of Anti-Cancer Drug In Human Lung Cancer Cells

抑制性寡脱氧核苷酸协同增强抗癌药物对人肺癌细胞的抗增殖作用

• 发表时间: 2011
• 作者: Takahashi R;Sato T;Miyazawa N;Klinman DM;et al
• 通讯作者: et al

Serum Heme Oxygenase-1 as a Marker of Lung Function Decline in Patients With Chronic Silicosis

• DOI: 10.1097/jom.0b013e3182636e93
• 发表时间: 2012-12-01
• 期刊: JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE
• 影响因子: 3.2
• 作者: Sato, Takashi;Saito, Yoshiaki;Ishigatsubo, Yoshiaki
• 通讯作者: Ishigatsubo, Yoshiaki