Research for biologically active saponins from Japanese and Chinese herbal medicine on the basis of their traditional use.

在传统用途的基础上研究日本和中国草药的生物活性皂苷。

• 批准号: 09672177
• 负责人: MATSUDA Hisashi
• 金额: $ 1.98万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672177/
• 关键词: elucidation of traditional medicine; bilogically active saponin; Japanese and Chinese herbal medicine; glucose absorption; immune response; hepatoprotective activity; structure-activity relationship; action mechanism; 柴胡; 西洋トチノキ種子; 抗アレルギー作用; アジ

The effects of various Japanese and Chinese herbal medicines on glucose absorption, allergy, immune responses. and hepatic injury were examined.From the the leaves of Gymnema sylvestre, gimnemosides a-f were isolated and their structures were determined on the basis of chemical and physicochemical evidence. These saponins inhibited both increase of blood glucose levels in oral glucose-loaded rats and glucose uptake at small intestinal brush borders. Several oleanolic acid glycosides potently inhibited the increased blood glucose levels in oral glucose-loaded rats. To clarify the structural requirements of oleanolic acid glycosides, we examined the inhibitory activity of various oleanolic acid glycosides isolated from Momordica cochinchinensis, Kochia scoparia, Panax japonicus, etc. The results revealed that the 3-O-monodesmoside and 28-carboxy group are essential for such activity. Furthermore, investigation of the action mechanism revealed that they inhibit gastric emptying and glucos … More e absorption at small intestinal brush borders, and that capsaicin-sensitive sensory nerves, the sympathetic nervous system, the central nervous systems, and central dopamine2 receptors are involved in the inhibition of gastric emptying.Sandosaponins A and B with potent histamine release inhibitory activity were isolated from kidney bean (Phaseolus vulgaris). Labolabosides A-F from hyacinth bean (Dolichos lablab), jujubosides from Zizyphi Spinosi Semen (Zizyphus jujuba var. spinosa), and soyasaponins from several beans found to show adjuvant-like activity in mice. The chemical structures of twelve new saponines (escins and isolescins), isolated from horse chestnuts (Aesculus hippocatanum), were determined. Escins Ia-IIb, found to show antiinfiammatory effects in rats and mice As concerns their hypoglycemic activities, it was revealed that their action mechanisms are similar to those of oleanolic acid glycosides,The chemical structures of qunquenosides I-III from American ginseng (Panax quiquefoliwn), and bupleurosides I-XIII and scorzonerosides A*C with hepatoprotective activity from Chinese Bupleuri Radix (Bupleurum scorzonerifolium) were determined. Strucural requirements of bupleurosides for hepatoprotective activity were clarified. Less

各种日本和中国草药对葡萄糖吸收，过敏，免疫反应的影响。从匙羹藤（Gymnema sylvestre）的叶中分离得到gimnemosides a-f，并根据化学和物理化学证据确定了它们的结构。这些皂苷抑制口服葡萄糖负荷大鼠血糖水平的升高和小肠刷状缘的葡萄糖摄取。几种油酸糖苷可有效抑制口服葡萄糖负荷大鼠的血糖水平升高。为了阐明齐墩果酸苷的结构要求，我们研究了从木麻黄、地肤、人参等中分离的各种齐墩果酸苷的抑制活性。结果表明，3-O-单桥糖甙和28-羧基是这种活性所必需的。其作用机制的研究表明，它们能抑制胃排空， ...更多信息 从菜豆（Phaseolus vulgaris）中分离得到具有抑制组胺释放活性山参皂苷A和B，并与辣椒素敏感的感觉神经、交感神经系统、中枢神经系统和中枢多巴胺2受体有关。扁豆（Dolichos lablab）的Labolabosides A-F、酸枣仁（Zizyphus Spinosi Semen）的Labolabosides A-F、酸枣仁（Zizyphus spinba var. spinosa），以及来自几种豆类的大豆皂苷，发现它们在小鼠中显示出类似于泻药的活性。从七叶树（Aesculus spiccatanum）中分离得到12个新的皂苷类化合物（七叶皂苷和异七叶皂苷）。七叶皂苷Ⅰ a-Ⅱ b，在大鼠和小鼠体内均具有抗炎作用，其降血糖作用机制与油酸苷相似。测定了西洋参中的昆昆苷Ⅰ-Ⅲ和柴胡中具有保肝活性的柴胡皂苷Ⅰ-Ⅲ和狭叶皂苷A*C的化学结构。阐明了柴胡皂苷保肝活性的结构要求。少

项目成果

Hideaki Matsuda, Yue Dai, Yasuko Ido, Toshiyuki Murakami, Hisashi Matsuda, Masayuki Yoshikawa, and Michinori Kubo: "Studies on Kochiae Fructus.V.Antipruritic Effects of Oleanolic Acid Glycosides and the Structure-Requirement." Biol.Pharm.Bull.21. 1231-123

Hideaki Matsuda、Yue Dai、Yasuko Ido、Toshiyuki Murakami、Hisashi Matsuda、Masayuki Yoshikawa 和 Michinori Kubo：“地肤子的研究。V.齐墩果酸苷的止痒作用及其结构要求。”

Hisashi Matsuda, Toshiyuki Murakami, Narumi Matsumura, Masayuki Yoshikawa, Kenji Oda, Shigeji Katayama, and Toshiaki Ohgitani: "Effects of Saponins from Medicinal Foodstuffs on Immune Response-Ajuvant Activity of Soyasaponins-" J.Trad.Med.15. 278-279 (199

Hisashi Matsuda、Toshiyuki Murakami、Narumi Matsumura、Masayuki Yoshikawa、Kenji Oda、Shigeji Katayama 和 Toshiaki Ohgitani：“药用食品中的皂苷对免疫反应的影响 - 大豆皂苷的辅助活性 -”J.Trad.Med.15。

Hisashi Matsuda, Yuhao Li, and Masayuki Yoshikawa: "Roles of Capsaicin-Sensitive Sensory Nerves, Endogenous Nitric Oxide, Sulfthydryls, and Prostaglandins in Gastroprotection by Momordin Ic, and Oleanolic Acid Oligoglycoside, on Ethanol-Induced Gastric Mu

Hisashi Matsuda、Yuhao Li 和 Masayuki Yoshikawa：“辣椒素敏感的感觉神经、内源性一氧化氮、硫氢基和前列腺素在苦瓜苷 Ic 和齐墩果酸寡糖苷对乙醇诱导的胃粘膜保护中的作用

Masayuki Yoshikawa: "Absolute Stereostructures of Spinacosides C and D with a Novel Acetal Type Substituent from Spinacia oleracea (Spinach) and Basella rubra (Indian Spinach)." Heterocycles. vol.49. 93-96 (1998)

Masayuki Yoshikawa：“菠菜苷 C 和 D 的绝对立体结构以及来自 Spinacia oleracea（菠菜）和 Basella rubra（印度菠菜）的新型缩醛型取代基。”

Hisashi Matsuda: "Mode of Action of Escins Ia and IIa and E,Z-Senegin II on Glucose Absorption in Gastrointestinal Tract" Bioorganic & Medicinal Chemistry. Vol.6(in press). (1997)

Hisashi Matsuda：“七叶皂苷 Ia 和 IIa 以及 E,Z-Senegin II 对胃肠道葡萄糖吸收的作用模式”生物有机