Search for effective constituents for life-style related diseases through expression of PPAR

通过PPAR表达寻找生活方式相关疾病的有效成分

• 批准号: 22510240
• 负责人: MATSUDA Hisashi
• 金额: $ 2.83万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22510240/
• 关键词: アーユルベヴェーダ生薬; PPAR; メトキシフラボノール; アポルフィン型アルカロイド; アーユルヴェーダ生薬; 生薬; 生活習慣病; 糖尿病; 辛味成分; フラボノール; クロロゲン酸誘導体; PPARγ; 構造活性相関; piperlonguminine; Nulumbo nucifera

We mainly focused on Ayurvedic traditional medicine and have researched the active constituents for therapeutics of life-style related diseases through expression of peroxisome proliferator-activated receptor (PPAR). As the results, the extracts and the constituents from therhizomes of Kaempferia parviflora, flower of Nelumbo nucifera, and amide constituents from the fruit of Piper chaba, which are cultivated in India as well as southeast Asia, were found to show PPARγ agonist-like effects in 3T3-L1 adipocytes. The active constituents, 3,5,7,3’,4’-penta- mehoxyflavonol, N-nornuciferine, piperlonguminine, and their derivatives showed enhancement of PPARγ2, C/EBPα and β mRNA, although they had less agonistic activity at PPARγ levels. Furhermore,plasma concentrations were determined after administration of piperlonguminine and retrofractamide A in mice, together with synthesis of retrofactamide A and its derivatives to find more effectiveconstituents.

我们主要关注阿育吠陀传统医学，通过过氧化物酶体增殖激活受体（PPAR）的表达，研究了治疗生活方式相关疾病的有效成分。结果发现，产自印度和东南亚的山柰（Kaempferia parviflora）、莲花（Nelumbo nucifera）的therhi茎提取物和成分，以及沙巴胡椒（Piper chaba）果实的酰胺成分对3T3-L1脂肪细胞具有类似PPARγ激动剂的作用。活性成分3,5,7,3 ',4 ' -五-甲氧基黄酮醇，n -去甲花籽碱，胡椒明及其衍生物均能增强PPARγ2， C/EBPα和β mRNA，但对PPARγ水平的拮抗作用较弱。此外，在给药哌隆明和逆转录酶甲酰胺A后测定小鼠血浆浓度，并合成逆转录酶甲酰胺A及其衍生物，以寻找更有效的成分。

项目成果

フラボノイド類の脂肪細胞分化誘導活性.

类黄酮的脂肪细胞分化诱导活性。

• 发表时间: 2010
• 作者: Shimo T;Matsukubo H;Zhang H-M;松田久司,小神雄一郎,杉山智美,上野翼,中村誠宏,吉川雅之
• 通讯作者: 松田久司,小神雄一郎,杉山智美,上野翼,中村誠宏,吉川雅之

Adipogenic effects of piperlonguminine in 3T3-L1 cells and plasma concentrations of several amide constituents from Piper chaba extracts after treatment of mice

• DOI: 10.1007/s11418-013-0770-3
• 发表时间: 2013-04
• 期刊: Journal of Natural Medicines
• 影响因子: 3.3
• 作者: Itadaki Yamaguchi;H. Matsuda;Hailong Zhang;M. Hamao;Chihiro Yamashita;Yuichiro Kogami;Haruka Kon'i;Megumi Murata;Seikou Nakamura;M. Yoshikawa

Osamu Muraoka, Takao Hayakawa, Masayuki Yoshikawa: Promoting the effect of chemical constituents from the flowers of Poacynum hendersonii on adipogenesis in 3T3-L1 cells.

Osamu Muraoka、Takao Hayakawa、Masayuki Yoshikawa：促进 Poacynum hendersonii 花化学成分对 3T3-L1 细胞脂肪生成的影响。

• DOI: 10.1007/s11418-011-0549-3
• 发表时间: 2012
• 期刊: J. Nat. Med.
• 作者: Toshio Morikawa;Katsuya Imura;Sohachiro Miyake;Kiyofumi Ninomiya;Hisashi Matsuda;Chihiro Yamashita
• 通讯作者: Chihiro Yamashita

薬用食品から抗糖尿病作用成分の開拓に挑む.

试图从药用食品中发现抗糖尿病成分。

• 发表时间: 2011
• 期刊: Yakugaku Zasshi
• 影响因子: 0.3
• 作者: Ochi A;Abe S;Morita EH;中村誠宏,松田久司,吉川雅之
• 通讯作者: 中村誠宏,松田久司,吉川雅之

Effects of amide constituentsfrom pepper (Piper chaba, Fruit) on adipogenesis in 3T3-L1 cells.

胡椒（Piper chaba，水果）中的酰胺成分对 3T3-L1 细胞脂肪生成的影响。

• 发表时间: 2011
• 作者: Seikou Nakamura;Hisashi Matsuda;Chihiro Yamashita;Hailong Zhang;MasayukiYoshikawa
• 通讯作者: MasayukiYoshikawa