Molecular mechanisms of egg activation induced by RGD peptides.

RGD 肽诱导卵子活化的分子机制。

• 批准号: 09680726
• 负责人: IWAO Yasuhiro
• 金额: $ 2.05万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680726/
• 关键词: Fertilization; Amphibians; Sperm; Integrins; Ca ion; シグナルトランスダクション; caイオン; シグナルタランスダクション

We had found that RGD-containing peptides as well as the tryptic sperm protease induced egg activation in Xenopus. In this study, we have tried to identify the sperm agonist and the egg receptors in the process of egg activation.(1)An egg receptor for RGDS peptide was purified by several chromatographies with some columns including a RGD-conjugated columns. To clarify its molecular interactions with RGD peptides in vivo, the molecules which had been labelled by bi-phasic reagents with RGDS peptides were collected and then their molecular future was investigated.(2)On the cascade between sperm-egg binding and an increase in intra cellular Ca2+, Src-family trypsin kinase is involved in the activation process induced by both RGD peptides and fertilizing sperm.(3)A putative sperm agonist, an acrosomal protease, has been purified. It's molecular entities as well as the relationship with RGD peptides is investigated.(4)On the activation of urodele eggs, the relationship between sperm agonists and an intra cellular egg activation factor of sperm were investigated.

我们发现，含有RGD的肽以及胰蛋白酶精子蛋白酶诱导卵激活非洲爪蟾。本研究试图鉴定卵子激活过程中的精子激动剂和卵子受体。(1)An RGDS肽的卵受体通过几种色谱法纯化，其中一些柱包括RGD-缀合柱。为了阐明其在体内与RGD肽的分子相互作用，收集了用RGDS肽双相试剂标记的分子，然后对其分子未来进行了研究。(2)On精卵结合和细胞内Ca 2+，Src-家族胰蛋白酶激酶增加之间的级联反应参与了由RGD肽和精子诱导的激活过程。(3)A推测的精子激动剂，一种顶体蛋白酶，已经被纯化。探讨了其分子实体及其与RGD多肽的关系。(4)On研究了有尾目动物卵子的激活、精子激动剂与精子细胞内卵子激活因子的关系。

项目成果

SATO, K: "Evidence for the involvement of a Src-related tyrosine kinase in the Xenopus egg activation."Developmental Biology. 209. 308-320 (1999)

SATO, K：“Src 相关酪氨酸激酶参与非洲爪蟾卵激活的证据。”发育生物学。

S.Yamamoto: "The rise of intracellular Ca2+ level causes the decrease of cyclin B1 and Mos in the newt eggs at fertilization."Molecular Reproduction&Development. 53. 341-349 (1999)

S.Yamamoto：“细胞内 Ca2 水平的升高导致受精时蝾螈卵中细胞周期蛋白 B1 和 Mos 的减少。” 分子生殖

Ken-ichi Sato: "Evidence for the involvement of a Src-related tyrosine kinase in the Xenopus egg activation." Developmental Bilolgy. (印刷中).

Ken-ichi Sato：“Src 相关酪氨酸激酶参与爪蟾卵激活的证据”（正在出版）。

MIZOTE, A: "Activation of Xenopus eggs by proteases : Possible involvement of a sperm protease in fertilization."Developmental Biology. 208. 79-92 (1999)

MIZOTE，A：“蛋白酶激活非洲爪蟾卵：精子蛋白酶可能参与受精。”发育生物学。

Ichirou Sakamoto: "Changes in cyclin B during oocyte maturation and early embryonic cell cycle in the newt, Cynops pyrrhogaster. Recquirement of germinal vesicle for MPF activation" Developmental. (印刷中).

Ichirou Sakamoto：“蝾螈卵母细胞成熟和早期胚胎细胞周期中细胞周期蛋白 B 的变化。MPF 激活的生发囊泡的要求”（正在出版）。