Molecular mechanisms of egg activation induced by RGD peptides.

我们已经发现，含有RGD的多肽以及胰酶精子蛋白能诱导非洲爪哇的卵子激活。在本研究中，我们试图鉴定卵子激活过程中的精子激动剂和卵子受体。(1)用包括RGD偶联柱在内的几种柱层析纯化了RGDS多肽的卵子受体。为了阐明其在体内与RGD多肽的分子相互作用，收集了RGDS多肽双相试剂标记的分子，并对其分子前景进行了研究。(2)在精子-卵子结合和细胞内钙升高的级联反应中，Src家族胰蛋白酶参与了RGD多肽和受精精子诱导的激活过程。(3)分离纯化了一种可能的精子激动剂--顶体蛋白水解酶。研究了精子激动剂与精子胞内卵子激活因子的关系。

We had found that RGD-containing peptides as well as the tryptic sperm protease induced egg activation in Xenopus. In this study, we have tried to identify the sperm agonist and the egg receptors in the process of egg activation.(1)An egg receptor for RGDS peptide was purified by several chromatographies with some columns including a RGD-conjugated columns. To clarify its molecular interactions with RGD peptides in vivo, the molecules which had been labelled by bi-phasic reagents with RGDS peptides were collected and then their molecular future was investigated.(2)On the cascade between sperm-egg binding and an increase in intra cellular Ca2+, Src-family trypsin kinase is involved in the activation process induced by both RGD peptides and fertilizing sperm.(3)A putative sperm agonist, an acrosomal protease, has been purified. It's molecular entities as well as the relationship with RGD peptides is investigated.(4)On the activation of urodele eggs, the relationship between sperm agonists and an intra cellular egg activation factor of sperm were investigated.