B細胞終末分化の分子機構についての研究

为了确定Pax-5/BSAP在体内的功能，特别是在调节抗体分泌和B细胞分化中的功能，我们研究了Pax-5基因的基因组构型和B细胞中Pax-5启动子活性的特异性。此外，我们还创造了转基因小鼠，在其中Pax-5表达的生理调节被取消。为此，我们研究了Pax-5在B细胞向浆细胞分化过程中持续过表达的作用，即天然Pax-5表达被关闭的分化阶段。1.我们克隆了一段2kb的DNA片段，该片段跨越了Pax-5的外显子1A和5‘内含子区域。2、在该区域没有发现B细胞特异的调控元件。3、发现了调控Pax-5表达的新区域。4、发现了调控Pax-5表达的新转录因子。5、建立了Pax-5转基因小鼠。发现了意想不到的老鼠表型。该项目目前处于发表阶段。

In order to determine in vivo function of Pax-5/BSAP, function in the regulation of antibody secretion and B cell differentiation in particular, we investigated genomic configuration of Pax-5 gene and specificity of Pax-5 promotor activity in B cells. In addition, we have created transgenic mouse in which physiological regulation of Pax-5 expression is abrogated. By doing this, we studied the effect of sustained over expression of Pax-5 at the B cell differentiation to plasma cell, the differentiation stage at which native Pax-5 expression is turned off.1, We have cloned a DNA fragment of 2Kb which spans exon 1A of Pax-5 and 5' intronic region.2, No B cell specific regulatory elements were found in this region.3, Novel region regulating B cell specific expression of Pax-5 is identified.4, Novel transcription factors regulating Pax-5 expression was discovered.5, Pax-5 transgenic mice were established. Unexpected phenotype of the mice was identified.The project is now at the stage of publication.