B細胞終末分化の分子機構についての研究

为了确定Pax-5/BSAP在体内的功能，特别是在调节抗体分泌和B细胞分化中的功能，我们研究了Pax-5基因的基因组结构和B细胞中Pax-5启动子活性的特异性。此外，我们已经创建了转基因小鼠，其中Pax-5的生理调控被废除。通过这样做，我们研究了Pax-5持续过表达在B细胞向浆细胞分化的影响，在浆细胞分化阶段，原生Pax-5的表达被关闭。我们克隆了一个2Kb的DNA片段，该片段横跨Pax-5的外显子1A和5'内含子区。2、该区域未发现B细胞特异性调控元件。3、发现了B细胞特异性表达Pax-5的新区域。4、发现了新的调控Pax-5表达的转录因子。5、建立Pax-5转基因小鼠。发现了意想不到的小鼠表型。该项目目前正处于出版阶段。

In order to determine in vivo function of Pax-5/BSAP, function in the regulation of antibody secretion and B cell differentiation in particular, we investigated genomic configuration of Pax-5 gene and specificity of Pax-5 promotor activity in B cells. In addition, we have created transgenic mouse in which physiological regulation of Pax-5 expression is abrogated. By doing this, we studied the effect of sustained over expression of Pax-5 at the B cell differentiation to plasma cell, the differentiation stage at which native Pax-5 expression is turned off.1, We have cloned a DNA fragment of 2Kb which spans exon 1A of Pax-5 and 5' intronic region.2, No B cell specific regulatory elements were found in this region.3, Novel region regulating B cell specific expression of Pax-5 is identified.4, Novel transcription factors regulating Pax-5 expression was discovered.5, Pax-5 transgenic mice were established. Unexpected phenotype of the mice was identified.The project is now at the stage of publication.