Phase Transitions in the Higher Order Structure of Giant DNA Single Molecular Chain

巨型DNA单分子链高阶结构的相变

• 批准号: 10044073
• 负责人: YOSHIKAWA Kenichi
• 金额: $ 2.69万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044073/
• 关键词: Giant DNA; Coil-Globule Trancition; Single Chain Observation; Flurescente Microscopy; Single-Chain Folding; Melting Transitions; Surfactant; Folding Transition; コイルーグロビュール転移; コイル-グロビュール転移; 単分子観察; ミセル

The main purpose of the present research project was to study the folding transitions of long polymer chain, especially of giant DNA molecules. We have found that the conformation of giant DNAs is controlled by the addition of various kinds of surfactant. With the adjustment of alcohol concentrations, both the conformation and solvability are controlled (see e. g.; J. Am. Chem. Soc., 121, 1780-1785, 1999). It has been also found that the melting, or helix-coil transition, and the folding transition exhibit the cross-talk each other. When DNA is folded, melting transition is tendid to be prevented. When melting transition is generated, the unfolding of DNA is accelerated.

本课题的主要目的是研究高分子长链，特别是DNA大分子的折叠转变。我们发现大分子DNA的构象是由各种表面活性剂的加入所控制的。随着醇浓度的调整，构象和溶解性都得到控制（见e。g.; J. Am.化学会，121，1780-1785，1999）。还发现，熔融或螺旋-卷曲转变和折叠转变彼此表现出串扰。当DNA折叠时，解链转变倾向于被阻止。当发生解链转变时，DNA的解折叠加速。

项目成果

Sergeyev,V.G.: "How Does Alcohol Dissolve the Complex of DNA with Cationic Surfactant?"J. Am. Chem. Soc.. 121. 1780-1785 (1999)

Sergeyev,V.G.：“酒精如何溶解 DNA 与阳离子表面活性剂的复合物？”J.

Matsuzawa,Y.: "Laser Trapping of an Individual DNA Molecule Folded Using Various Condensing Agents"J. Am. Chem. Soc.. 121. 11581-11582 (1999)

Matsuzawa,Y.：“使用各种凝聚剂折叠的单个 DNA 分子的激光捕获”J。

Nomura,S.: "Giant Phospholiqid Vesicles Entrapping Giant DNA"Giant Vesicles. 313-317 (1999)

Nomura,S.：“巨型磷脂囊泡捕获巨型DNA”巨型囊泡。

S.Kidaki: "Folding and unfolding of a giant duplex-DNA ina mixed solution with polycations,polyanions and crowding neutral polymers" Biophys.Chem.109. 133-143 (1998)

S.Kidaki：“在聚阳离子、聚阴离子和拥挤中性聚合物的混合溶液中折叠和展开巨型双链 DNA”Biophys.Chem.109。

Ichiba,Y.: "Single Chain Observation on Collapse Transition in Giant DNA Induces by Negatively-Charged Polymer"Biochem. Biophys. Res. Commun.. 242. 441-445 (1998)

Ichiba,Y.：“负电荷聚合物诱导的巨型 DNA 塌陷转变的单链观察”Biochem。