课题基金 / 基金详情

Structure and functions of a very small tetraheme protein -Molecular mechanism of bioremediation-

Structure and functions of a very small tetraheme protein -Molecular mechanism of bioremediation-
极小的四血红素蛋白的结构和功能-生物修复的分子机制-
批准号:
10044072
负责人:
AKUTSU Hideo
金额:
$1.86万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B).
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 1999

项目摘要

项目成果

AKUTSU Hideo的其他基金

相关文献

中文摘要
翻译
many Shewanella species of many Shewanella species is their ability to reduce insolublemetal oxides, including ironmanganese. S. putrefaciens strain MR1 was specifically isolated as a metal oxide (iron andmanganese reducing organismand shown to couple the reduction of metal oxides to the oxidation of organic carbon the ability ofShewanella species to grow with a variety of different electron acceptors having redox potentialsfrom +0.8 V (oxygen) to about 0 (elemental sulfur) implies that this bacterium is able to just togrowth conditions through changes in the activity and/or the composition of the electron transportchain. Cytochromes are abundant in Shewanella and have been implicated in the reduction of metaloxides. The redox behavior of a tetraheme protein,cytochrome c - D23 - D2 was investigated in this work. The macroscopic and microscopic redoxpotentials were determined at pH 9. Although they are low in comparison with其他cytochromes,they are higher than the redox potentials of cytochrome c D23 D2 from Desulfovibrio vulgarisMiyazaki F (DvMF). DvMF cytochrome c - D23 - D2 gene was successfully expressed in a facultativeaerobe, Shewanella putrefaciens MR-I, under anaerobic, microaerobic,aerobic conditions with good yields. A derivative of the broad-host-range plasmid pRK415containing the cytochrome c - D23 - D2,genes from DvMF was used for transformation of S. putrefaciens MR-1resulting in the production of holocytochrome c - D23 - D2 that was indistinguishable from thatproduced by D. vulgaris Miyazaki
英文摘要
The most remarkable characteristic of many Shewanella species is their ability to reduce insoluble metal oxides, including iron, and manganese. S. putrefaciens strain MR1 was specifically isolated as a metal oxide (iron and manganese) reducing organism, and shown to couple the reduction of metal oxides to the oxidation of organic carbon. The ability of Shewanella species to grow with a variety of different electron acceptors having redox potentials from +0.8 V (oxygen) to about 0 (elemental sulfur) implies that this bacterium is able to adjust to growth conditions through changes in the activity and/or the composition of the electron transport chain. Cytochromes are abundant in Shewanella and have been implicated in the reduction of metal oxides. The redox behavior of a tetraheme protein, cytochrome cィイD23ィエD2 was investigated in this work. The macroscopic and microscopic redox potentials were determined at pH 9. Although they are low in comparison with other cytochromes, they are higher than the redox potentials of cytochrome cィイD23ィエD2 from Desulfovibrio vulgaris Miyazaki F (DvMF). DvMF cytochrome cィイD23ィエD2 gene was successfully expressed in a facultative aerobe, Shewanella putrefaciens MR-I, under anaerobic, microaerobic, and aerobic conditions with good yields. A derivative of the broad-host-range plasmid pRK415 containing the cytochrome cィイD23ィエD2, genes from DvMF was used for transformation of S. putrefaciens MR-1, resulting in the production of holocytochrome cィイD23ィエD2 that was indistinguishable from that produced by D. vulgaris Miyazaki F.
期刊论文(29)
专著(0)
科研奖励(0)
会议论文
T.Ohmura: "Ionic-strength dependent physicochemical factors in cytochrome c_3 regulating the electron transfer rate." Biophys.J.75. 1483-1490 (1998)
T.Ohmura:“细胞色素 c_3 中离子强度依赖性物理化学因素调节电子转移速率。”
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通讯作者:
T. Ohmura, E. Harada, T. Fujiwara, G. Kawai, K. Watanabe and H. Akutsu: "Paramagnetic Inversion of the sign of the interference contribution to the transverse relaxation of the imido protons of the coordinated imidazoles in the uniformly ィイD115ィエD1N label
T. Ohmura、E. Harada、T. Fujiwara、G. Kawai、K. Watanabe 和 H. Akutsu:“顺磁反转对均匀 D115 中配位咪唑的亚氨基质子横向弛豫的干扰贡献符号D1N标签
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S. Yoshioka: "Molecular mobility of protein in lyophilized formulations linked to the molecular mobility of polymer excipients, as determined by high resolution solid-state ^<13>C NMR"Pharmaceutical Res.. 16. 1621-1625 (1999)
S. Yoshioka:“通过高分辨率固态 13 C NMR 测定,冻干制剂中蛋白质的分子迁移率与聚合物赋形剂的分子迁移率相关”,Pharmaceutical Res.. 16. 1621-1625 (1999)
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29
    Molecular soft interactions regulating membrane-interface activities in living systems
    • 批准号:
      15083101
    • 项目类别:
      Grant-in-Aid for Scientific Research on Priority Areas
    • 资助金额:
      $61.25万
    • 财政年份:
      2003
    • 负责人:
      AKUTSU Hideo
    • 依托单位:
    Intermolecular soft interactions regulating H^+-ATPsynthase function
    • 批准号:
      15083203
    • 项目类别:
      Grant-in-Aid for Scientific Research on Priority Areas
    • 资助金额:
      $158.08万
    • 财政年份:
      2003
    • 负责人:
      AKUTSU Hideo
    • 依托单位:
    Rotary catalysis mechanism of H^+-ATP synthase investigated by novel NMR methodology
    • 批准号:
      14208082
    • 项目类别:
      Grant-in-Aid for Scientific Research (A)
    • 资助金额:
      $31.03万
    • 财政年份:
      2002
    • 负责人:
      AKUTSU Hideo
    • 依托单位:
    Development of New Methodologies for Protein-Interaction Analysis