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Investigation on the dynamic structure of the lipid-containing virus PM2 and correlation with the infectivity.

含脂病毒PM2的动态结构及其与感染力的相关性研究

基本信息

批准号：
60580221
负责人：
AKUTSU Hideo
金额：
$ 1.41万
依托单位：
Yokohama National University (1986)Osaka University (1985)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60580221/
关键词：
Bacteriophage PM2 DNA lipid bilayer biosupramolecular system <^(31)P> -NMR solid-state NMR 固体NMR

项目摘要

This project has two major aims. One is to establish a new NMR method which enable us to investigate supramolecular biological systems in vivo. The other one is to apply this method to a lipid-containing bacteriophage PM2 and its host cell, Alteromnas espejiana BAL-31. Such investigation would provide us the information on the dynamic structures of the intact PM2 and BAL-31. The fist aim has been almost accomplished. A part of the work was already published. Through this work it has been shown that the cross-polarization NMR is one of the powerful methods to investigate huge molecular complexes in vivo. In the second part of the project, the <^(31)P> -NMR spectra of lipid bilayers and nucleic acids in vivo were obtained separately not only for PM2 but also for the host cell, BAL-31 by using the cross-polarization NMR. The thermal properties of the multilamellar of the lipids extracted from PM2 and BAL-31 were examined with the Privalov type calorimeter DACM4. The phase transition behavior of the intact biomembranes and extracted lipids were compared. The termination temperature of the phase transition of the intact membranes was found to be lower for BAL-31 and higher for PM2 than that of the extracted lipids. The origin of the difference is not yet clear. The correlation between the dynamic structures and the infectivity was discussed on the basis of these data. It is suggested that the lipid bilayers of PM2 should be in the liquid-crystalline state for the effective infection.

该项目有两个主要目标。一是建立一种新的核磁共振方法，使我们能够在体内研究超分子生物体系。另一个是将该方法应用于含脂质噬菌体PM 2及其宿主细胞Alteromnas espejiana BAL-31。这样的调查将为我们提供完整的PM 2和BAL-31的动态结构的信息。第一个目标几乎已经实现。部分作品已经出版。通过这一工作表明，交叉极化核磁共振是在体内研究大分子复合物的有力手段之一。第二部分利用交叉极化核磁共振技术，分别获得了PM 2和BAL-31细胞内脂双层和核酸的<^（31）P> -核磁共振谱。用Privalov型量热计DACM 4检查从PM 2和BAL-31中提取的脂质的多层的热性质。比较了完整生物膜和提取脂质的相变行为。完整膜的相变的终止温度被认为是较低的BAL-31和较高的PM2比提取的脂质。差异的起源尚不清楚。在此基础上，讨论了其动态结构与感染性的关系。这表明，PM2的脂质双分子层应处于液晶状态，有效的感染。