Confocal laser scanning microscope system for live cell imaging

用于最高分辨率活细胞成像的共聚焦激光扫描显微镜（CLSM）系统将使申请人的研究小组能够用实验方法回答动物学中的广泛问题。一个主要的重点将是3D和4D记录的生理和发育的生物过程中的生活对象。将研究这些过程的动物类群多种多样，包括海洋和陆地无脊椎动物及其胚胎发生、神经系统中的神经生理过程以及组织切片和整体样品中分子特征的记录。另一个重点是亚细胞结构的记录，这些结构用荧光染料标记，因此以超分辨率重新编码。CLSM将是进行这些研究的理想和多功能工具。特别是对象的多样性使得系统具有很强的适应性。速度、高灵敏度和分辨率是执行计划项目的关键标准。该系统允许部分重叠的发射光谱的高度多路复用，以尽可能低的曝光量快速扫描样品，以免损坏敏感物体。特殊的检测器可以实现超分辨率，易于使用的软件可以自动找到样品并调整感兴趣的区域和每个荧光团的最佳照明。只有这些特征，早期胚胎阶段（2细胞或4细胞）才能以四维方式记录。该系统的主要用户是动物学和进化研究所的4个研究小组，但耶拿大学的其他研究人员也可以使用。该系统将用于启动研究小组之间的跨学科项目。该系统将增强FSU Jena的科学领域的频谱，并将动物学，神经科学，发育生物学等领域显着连接起来。

The Confocal-Laser-Scanning Microscope (CLSM) system for live-cell imaging with highest resolution will allow the research groups of the applicants to answer a broad spectrum of questions in zoology with experimental approaches. A major focus will be on the 3D- and 4D- recording of physiological and developmental biological processes in the living objects. The animal taxa in which these processes will be studied are diverse and include marine and terrestrial invertebrates and their embryogenesis, neurophysiological processes in the nervous system and the documentation of molecular signatures in histological sections and whole-mount samples. An additional focus is the documentation of sub-cellular structures, which were labelled with fluorescent dyes and are therefore recoded at super-resolution. The CLSM will be the ideal and versatile instrument to conduct these studies. Especially the large diversity of objects makes the system highly adaptable. Speed, high sensitivity, and resolution are key criteria to conduct the planned projects. The system allows high multiplexing of partly overlapping emission spectra, a fast scanning of the samples with the lowest light exposure as possible to not damage the sensitive objects. Special detectors allow super-resolution, and the easy-to-use software allows to automatically find the sample and adjust the region of interest and the optimal illumination of each fluorophore. Only with these features, early embryonic stages (2-cell or 4-cell) can be recorded in a 4-dimensional manner. The main user of the system are 4 research groups at the Institute for Zoology and Evolutionary Research but are also accessible for other researchers at the University of Jena. The system will be used to start interdisciplinary projects between the research group. The system will enhance the spectrum of scientific areas at the FSU Jena and connect the areas zoology, neuroscience, developmental biology significantly.