Production of Useful Chemicals from Glycerol by Metabolic Modification of Microorganisms

通过微生物代谢修饰从甘油生产有用化学品

• 批准号: 22KJ0427
• 负责人: ALAWI ASEEL
• 金额: $ 1.6万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ0427/
• 关键词: glycerol; 1,3-propanediol; 3-hydroxypropionic acid; Citrobacter braakii

To produce 3-hydroxypropionic acid (3-HP) from glycerol we used Citrobacter braakii strain TB-96-dLF, which was previously modified to produce high 1,3-propandiol (1,3-PD) concentration from glycerol. First, we disrupted propanediol dehydrogenase (pdh) gene to block 1,3-PD production (TB-96-dLFP). Since TB-96-dLFP may accumulate the toxic 3-hydroxypropionaldehyde (3-HPA) intermediate, we fermented it under aerobic condition as to produce 3-HP from glycerol via 3-HPA. However, no production of 3-HP was observed. This may be due to the instability of the first enzyme of glycerol metabolism which is glycerol dehydratase (Gdh) under aerobic conditions.

为了产生甘油中的3-羟基丙酸（3-HP），我们使用了柠檬酸杆菌菌株TB-96-DLF，以前修改以从甘油中产生高1,3-丙二醇（1,3-PD）浓度。首先，我们破坏了丙二醇脱氢酶（PDH）基因以阻断1,3-PD的产生（TB-96-DLFP）。由于TB-96-DLFP可能会积累有毒的3-羟基丙醛（3-HPA）中级，因此我们在有氧条件下发酵，以通过3-HPA从甘油中产生3-HP。但是，未观察到3-HP的产生。这可能是由于在有氧条件下是甘油脱水酶（GDH）的第一酶的不稳定。

项目成果

Metabolic Engineering of Citrobacter braakii strain TB-96 to Produce Useful Chemicals from Glycerol

布氏柠檬酸杆菌菌株 TB-96 的代谢工程从甘油生产有用的化学品

• 发表时间: 2022
• 作者: Aseel Alawi; Takahiro Yamaguchi;Toshiaki Nakajima-Kambe.
• 通讯作者: Toshiaki Nakajima-Kambe.

Metabolic engineering of a newly isolated Citrobacter braakii strain to produce 1,3-propanediol from glycerol

新分离的布氏柠檬酸杆菌菌株从甘油生产 1,3-丙二醇的代谢工程

• DOI: 10.1016/j.biteb.2022.101271
• 发表时间: 2022
• 期刊: Bioresource Technology Reports
• 作者: Alawi Aseel;Kiyoshi Keiji;Matsumoto Mizuki;Yamaguchi Takahiro;Narita Takuma;Morita Takahide;Suzuki Toshihiro;Nakajima-Kambe Toshiaki
• 通讯作者: Nakajima-Kambe Toshiaki