Production of Useful Chemicals from Glycerol by Metabolic Modification of Microorganisms

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To produce 3-hydroxypropionic acid (3-HP) from glycerol we used Citrobacter braakii strain TB-96-dLF, which was previously modified to produce high 1,3-propandiol (1,3-PD) concentration from glycerol. First, we disrupted propanediol dehydrogenase (pdh) gene to block 1,3-PD production (TB-96-dLFP). Since TB-96-dLFP may accumulate the toxic 3-hydroxypropionaldehyde (3-HPA) intermediate, we fermented it under aerobic condition as to produce 3-HP from glycerol via 3-HPA. However, no production of 3-HP was observed. This may be due to the instability of the first enzyme of glycerol metabolism which is glycerol dehydratase (Gdh) under aerobic conditions.