MOLECULAR EVALUATION OF 2,2-Bis(bromomethyl)-1,3-Propanediol(BMP) B6C3F1 Mouse

工作总结：检测到的唯一K-ras突变位于密码子12（GGT至GAT），并且在暴露浓度为312、625和1，250 ppm BMP的一半肿瘤和饲料对照中鉴定出。 我们的研究表明，K-ras基因的突变参与B6 C3 F1肺癌发生后BMP曝光。 基于对K-ras基因突变的检测，以及最近发现的定位于6号染色体远端K-ras基因区域的一个主要肺易感基因座，我们正在检测肺肿瘤远端6号染色体上的杂合性丢失（洛）。 迄今为止，已在BPM诱导的小鼠肺肿瘤中检测到6号染色体上的等位基因不平衡。 这些研究已经扩展到包括臭氧诱发肿瘤的评价。 还正在评价BMP诱导的哈氏腺肿瘤。预计将于2001年出版。

Summary of Work: The only K-ras mutation detected was at codon 12 (GGT to GAT), and was identified in half of the neoplasms from exposure concentrations of 312, 625 and 1,250 ppm BMP and in feed controls. Our study suggests that mutations in the K-ras gene are involved in B6C3F1 lung carcinogenesis following BMP exposure. Based on the detection of the K-ras mutation, and the recent identification of a major lung susceptibility locus mapped to the distal part of chromosome 6 in the region of the K-ras gene, we are examining the lung tumors for loss of heterozygosity (LOH) on distal chromosome 6. To date allelic imbalances on chromosome 6 have been detected in BPM induced mouse lung neoplasms. These studies have been extended to include evaluation of ozone induced neoplasms. Also being evaluated are BMP induced Harderian gland neoplasms. Publication is expected in 2001.