The multiplexed diagnosis of arbovirus infections using combinatorial probes

使用组合探针对虫媒病毒感染进行多重诊断

• 批准号: LP0454228
• 负责人: Dr Mark Gibbs
• 金额: $ 18.63万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-09-30 至 2007-07-05
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0454228
• 关键词: multiplexed; diagnosis; arbovirus; infections; using

Viruses that cause serious diseases such as hemorrhagic fever or encephalitis must be quickly identified. Diagnostic tests based on DNA hybridisation are accurate and can be rapid but they are expensive. We will test a method for simplifying DNA tests and increasing their capabilities. DNA probes for detecting arboviruses will be designed at the ANU using new bioinformatic methods and their reliability will be modelled using all the available genetic information. Computer predictions will be experimentally tested in the PANBIO laboratory by using the probes to detect viral nucleic acids. The influence of virus genome complexity will be investigated

引起严重疾病如出血热或脑炎的病毒必须迅速查明。 基于DNA杂交的诊断测试是准确的，可以是快速的，但它们是昂贵的。 我们将测试一种简化DNA测试并提高其能力的方法。 ANU将使用新的生物信息学方法设计用于检测虫媒病毒的DNA探针，并将使用所有可用的遗传信息对其可靠性进行建模。 计算机预测将在PANBIO实验室通过使用探针检测病毒核酸进行实验测试。 将研究病毒基因组复杂性的影响