University of Washington Arboviral Research Network (UWARN)

华盛顿大学虫媒病毒研究网络 (UWARN)

• 批准号: 10687434
• 负责人: Michael Gale
• 金额: $ 116.6万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10687434
• 关键词: Acute; Address; Administrative Coordination; Alphavirus; Animals; Antibodies; Arbovirus Infections; Arboviruses; Area; Arthropod Vectors; Back; Bacteriology; Binding; Biological Assay; Biological Markers; Blood; Body Fluids; Brazil; Cell Separation; Chikungunya virus; Clinical; Clinical Management; Clinical Trials; Collaborations; Collection; Congo; Containment; Data; Dengue; Dengue Infection; Dengue Virus; Detection; Development; Diagnosis; Diagnostic; Diagnostic Reagent; Disease; Emergency Situation; Emerging Communicable Diseases; Engineering; Enzyme-Linked Immunosorbent Assay; Epitopes; Far East; Fever; Flavivirus; Fluorescence; Freezing; Future; Genomic approach; Human; Immune response; Immune system; Immunoglobulin G; Individual; Infection; Infectious Diseases Research; Innate Immune Response; Innate Immune System; Institutes; Institution; International; Intervention; Investigation; Japanese Encephalitis; Knowledge; Laboratories; Laboratory Research; Lead; Light; Logistics; Measurement; Metagenomics; Microcephaly; Molecular; Monitor; Monoclonal Antibodies; Natural Immunity; Oropouche virus; Orthobunyavirus; Outcome; Pakistan; Parasitic infection; Pathogenesis; Pathway interactions; Patients; Population; Prevalence; Process; Production; Protein Engineering; Proteins; Readiness; Reagent; Research; Resources; Rift Valley Fever; Risk; Sampling; Senegal; Sensitivity and Specificity; Serology; Seroprevalences; Site; South Africa; South America; Specimen; Statistical Data Interpretation; System; Taiwan; Technology; Technology Transfer; Testing; The science of Mycology; Therapeutic; Time; Universities; Vaccines; Variant; Veterinarians; Viral; Viral Antibodies; Viral Hemorrhagic Fevers; Viral Load result; Virus; Virus Diseases; Washington; Yellow Fever; ZIKA; Zika Virus; antagonist; arthropod-borne; base; biobank; candidate marker; chikungunya; cohort; cross reactivity; data management; design; experience; global health; global health emergency; human monoclonal antibodies; improved; improved outcome; innovation; neutralizing antibody; neutralizing monoclonal antibodies; next generation sequencing; novel; novel diagnostics; novel strategies; pandemic disease; pandemic preparedness; pathogen; pathogenic virus; point of care; population based; prospective; severe dengue; therapeutic target; transcriptomics; viral detection; virology; virus host interaction

Abstract: University of Washington Arboviral Research Network (UWARN) The University of Washington Arboviral Research Network (UWARN) will address emerging and re-emerging infectious diseases by carrying out research on arboviruses that include current high burden pathogens including dengue, chikungunya, and Zika viruses. Arboviruses also include emerging and re-emerging viruses such as Mayaro, Una, Usutu, Japanese encephalitis, yellow fever, Crimean-Congo hemorrhagic fever, and Oropouche viruses. UWARN will leverage the UW Metacenter for Pandemic Disease Preparedness and strong research laboratory partners in Brazil, Senegal, South Africa, Pakistan and Taiwan as well as the UW Department of Global Health global reach in capacity building. The UWARN international Collaborating Partners have institution-based and population-based cohorts in place to carry out the planned research and monitor for new virus emergence. The proposed UWARN research will create new human viral-neutralizing monoclonal antibodies (Hu-nMabs) that can be used as therapeutics or diagnostics. UWARN research will also create new diagnostics, created ab initio by the Institute for Protein Design, as artificial proteins that release light when antibodies to virus are present in body fluids. Finally, UWARN research will generate an understanding of how viruses manipulate the human innate immune system, and this information will be used to design biomarkers to predict severe disease as well as to suggest host-directed therapies that could lead to better outcomes after arboviral infection. The proposed technologies are generic and could be rapidly adapted to any future emerging arbovirus threat. UWARN collaborators span virology, bacteriology, mycology, and parasitic infections, such that UWARN can respond to diverse emerging infectious diseases threats. UWARN international laboratories have advanced capabilities including biorepositories with generator-power backed-up -80C freezers, next-generation sequencing (NGS) capabilities, fluorescence cell sorting, and BSL-3 containment facilities. UWARN cohorts have biobanked samples taken from individuals during acute febrile illnesses, with known and unknown arboviral diseases, and partnership sites are poised for prospective collection of population and facility–based samples. UWARN labs will use metagenomic NGS to detect novel viral emergence in affiliated cohorts. UWARN Partners have collaborating entomologists and veterinarians to sample arthropod vectors and animal reservoirs as needed. Additional innovative capacity included the Brazil UWARN partner’s mobile van with on board NGS capability for real-time, on-site viral sequencing and sampling to viral identification in less than 18 hrs. A second partner, IRESSEF in Senegal, plans to set up a similar mobile vehicle for viral sampling and sequencing in Senegal and the Brazil group will collaborate with IRESSEF on technology transfer and best practices. UWARN partners will serve as a resource to other Emerging Infectious Diseases Research Centers (EIDRC) providing expertise in monoclonal antibodies, protein design, and host directed therapies.

摘要：华盛顿大学虫媒病毒研究网络（UWARN）