Physiological, morphological and ecological studies on the multiplication of red tide organisms

赤潮生物繁殖的生理、形态和生态研究

• 批准号: 59390002
• 负责人: FUJII Tadashi
• 金额: $ 5.12万
• 依托单位: TSUKUBA University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59390002/
• 关键词: Carbon metabolism; Cell division; Chloroplast replication; Diurnal vertical migration; Heterosigma akashiwo; Nitrogen metabolism

1)Taxonomical studies : We have clarified the taxonomic confusions as follows: a) the algae described under the name of Olisthodiscus luteus by many phocologists is identical to H. akashiwo. b) Fibrocapsa japonica must be treated with an autonomous genus separated from Chattonella.2)Cell division and chloroplast replication : The cell division and chloroplast replication in Heterosigma akashiwo occurred as separate synchronous events during the cell cycle, when the cells were subjected to light-dark regimes. The synchronization index of cell division in cells cultured under a 12h L/12h D was calculted to be 0.79 - 0.83, indicating the better synchronization. These results indicated that H. akashiwo cells are an excellent material for the analysis of various biological events. Changes in fine structures accompanying with the cell division and chloroplast replication were observed using electrom microscope.3) Diurnal vertical migration behavior : H. akashiwo cells clearly showed diurnal … More vertical migration. The upward migration commenced a few hours before the light turned on and the downward migration a few hours before it was turned off. The diurnal verical migration behavior was closely correlated with diurnal changes in the specific gravity of the cells and/or the accumulation of neutral fat on the side of the nucleus away from the flagellar bases. This behavior was also correlated with the nitrogen metabolism, the upward migration being lost under nitrogen starvation. These results indicated that H. akashiwo cells are an excellent material fot eht analysis of cell behaviors.4) Carbon and nitrogen metabolism : The rate of photosynthetic <CO_2> fixation in H. akashiwo was not inhibited by higher light intensities, The oxygen inhibition of photosynthesis was very small. Photosynthetic <CO_2> fixation in this algal cell was mainly carried out through the reductive pentose phosphate cycle and <beta> -1,3-glucan was the major storage product of photosynthesis. The diurnal variations in nitrate reductase activity were found, if the cells were cultured in N-rich medium, whereas such variations could not be detected in cells cultured in N-starved medium. The pattern of variation was well correlated with that of the diurnal vertical migration behavior. Less

1)分类学研究：我们澄清了分类学上的混淆：（1）许多植物学家称之为Olisthodiscus luteus的藻类与H.明石。B）日本纤维藻必须用从Chattonella中分离出来的一个独立的属来处理。2）细胞分裂和叶绿体复制：赤潮异弯藻的细胞分裂和叶绿体复制在细胞周期中是独立的同步事件，当细胞处于光-暗状态时。在12 h L/12 h D条件下培养的细胞分裂同步化指数为0.79 - 0.83，表明细胞分裂同步化较好。这些结果表明，H. Akashiwo细胞是用于分析各种生物事件的极好材料。电镜下观察到细胞分裂和叶绿体复制过程中伴随的细微结构变化。akashiwo细胞清楚地显示出昼夜变化 ...更多信息 垂直迁移向上迁移在灯打开前几小时开始，向下迁移在灯关闭前几小时开始。昼夜垂直迁移行为密切相关的细胞的比重和/或在远离鞭毛基地的核侧的中性脂肪的积累的昼夜变化。这种行为也与氮代谢有关，氮饥饿时向上迁移会丧失。这些结果表明，H.赤藻细胞是研究细胞行为的良好材料<CO_2>。高光强不抑制赤霉素的光合作用，氧对光合作用的抑制很小。该<CO_2>藻细胞的光合固定主要通过还原性戊糖磷酸循环进行，<beta>-1，3-葡聚糖是光合作用的主要储存产物。硝酸还原酶活性的昼夜变化，如果细胞培养在富氮培养基中，而这种变化不能检测到在N饥饿培养基中培养的细胞。其变化规律与昼夜垂直迁移规律有很好的相关性。少

项目成果

I.Inouye: Arch.Protistenk. 132. 313-324 (1986)

I.Inouye：Arch.Protistenk。

Hara, Y. and M. Chihara: "Ultrastructure and Taxonomy of Fibrocapsa japonica (Class Raphidophyceae)." Arch. Protistenk.130. 133-141 (1985)

Hara, Y. 和 M. Chihara：“Fibrocapsa japonica（Raphidophyceae）的超微结构和分类学”。

Wada, M., A. Miyazaki and T. Fujii: "On the Mechanisms of Vertical Migration Behavior of Heterosigma akashiwo" Plant Cell Physiol.,. 26. 431-436 (1985)

Wada, M.、A. Miyazaki 和 T. Fujii：“赤潮异形藻垂直迁移行为的机制”植物细胞生理学。

Y.Hara: Bot.Mag.Tokyo. 98. 251-262 (1985)

Y.Hara：Bot.Mag.Tokyo。

M.Wada: Plant Cell Physiol.26. 431-436 (1985)

M.Wada：植物细胞生理学.26。