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Fine structural and cytochemical aspects on the secretory and endocytotic activities of cells.

细胞分泌和内吞活性的精细结构和细胞化学方面。

基本信息

批准号：
59440021
负责人：
FUJITA Hisao
金额：
$ 19.2万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

项目摘要

1) When rat anterior pituitary cells were cultured in the medium containing phospholipase <A_2> activators, multigranular exocytosis were found.2) In the apical cytoplasm of the thyroid follicle epithelial cell, caldesmon and actin are demonstrated by immunocytochemistry. The thyroid cell activity for reabsorption of colloid, caldesmon and actin are considered to play a great role.3) After the basal lamina is digested by collagenase and trypsin, and the organ is cultured in the medium containing high concentration of fetal calf serum, the cell polarity of each follicle is reversed. This mechanism is detected by electron microscopy and freeze fracture method.4) Kupffer cells endocytosing numerous latex beads and India ink particles are gathered in the connective tissue and form granulomas. They stay there for a long time, perhaps for whole life of the animal. This is considered to be a special vital protecting reaction for non-toxic foreign materials.5) Aromatase which is the enzyme necessary for synthesize estrogen from testosterone was demonstrated in the theca interna cells of the ovary.

1)将大鼠垂体前叶细胞置于含有磷脂酶<A_2>激活剂的培养基中培养后，出现多颗粒胞外分泌。2)在甲状腺滤泡上皮细胞的顶端细胞质中，免疫细胞化学显示caldesmon和actin。甲状腺细胞对胶体、钙素和肌动蛋白的重吸收活性被认为起着重要的作用。3)经胶原酶和胰蛋白酶消化基底膜后，在含有高浓度胎牛血清的培养基中培养，各卵泡的细胞极性发生逆转。采用电子显微镜和冷冻断口法对其机理进行了研究。4)吞噬大量乳胶珠和墨汁颗粒的库普弗细胞聚集在结缔组织中形成肉芽肿。它们在那里停留很长时间，也许是动物的一生。这被认为是对无毒外来物质的一种特殊的重要保护反应。5)卵巢内膜细胞中存在由睾酮合成雌激素所必需的芳香化酶。