Studies on the Pulpal Responses to the Dental Materials Using Germfree Rats

使用无菌大鼠研究牙髓对牙科材料的反应

基本信息

批准号：
59440079
负责人：
KOBAYASHI Chihiro
金额：
$ 7.81万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

项目摘要

1. The response of the exposed pulp to composite resin was investigated in germfree rats. The results indicated that composite resin had an irritating potential especially to the circulatory systems of the rat molar pulp.2. Bacterial endotoxin was applied to the root canal of germfree rats and the toxic effects of endotoxin on periapical tissue were investigated. Resorption of the periapical alveolar bone was observed, but irritation of the endotoxin seemed to decrease by the formation of the periapical barrier consisting of dense connective tissue and cementum deposite, then the resorbed alveolar bone was regenerated.3. Perforated site of the floor of germfree rat molar pulp chamber were treated with various materials and the results were histopathologically evaluated. Inflammatory responses were weak in control and amalgam group, and severe in Ca <(OH)_2> , hydroxyapatite and zinc oxide eugenol cement group. Control and hydroxyapatite group seemed to have some possibility of sealing the perforated site with the calcified tissue.4. Experiments were performed to make a model of pulpitis following periapical lesion under an aseptic condition using conventional rats. To necrotize the pulp, thermal injury by soldering iron or mechanical injury by luxation was applied to the rat molar. Injured pulps were necrotized, then entire pulp chambers were filled with osteo-dentin, although periapical inflammation was not observed in both experimental groups.

1。在无用的大鼠中研究了暴露的果肉对复合树脂的反应。结果表明，复合树脂具有刺激性的潜力，尤其是对大鼠摩尔纸浆的循环系统2。将细菌性内毒素应用于无毛虫大鼠的根管，并研究了内毒素对根尖组织组织的毒性作用。观察到根尖的牙槽骨骨的吸收，但是内毒素的刺激似乎通过形成由密集的结缔组织和牙骨质沉积物组成的根尖屏障的形成减少，然后再生肺泡骨。3。用各种材料处理了无毛大鼠摩尔浆腔室的底部的穿孔部位，并对结果进行了组织病理学评估。炎症反应在对照组和汞齐组中较弱，在Ca <（OH）_2>，羟基磷灰石和氧化氧化物Eugenol水泥组中严重。对照和羟基磷灰石组似乎有可能用钙化组织密封穿孔位点。4。使用常规大鼠在无菌疾病下进行根尖细胞病变后进行牙髓炎模型。为了使果肉坏死，通过将铁或机械损伤焊接到大鼠磨牙上。将受伤的牙髓坏死，然后将整个纸浆腔填充骨丁丁，尽管在两个实验组中均未观察到根尖的炎症。