Studies of vascular system based on cell biological, molecular biological and metabolic approaches.

基于细胞生物学、分子生物学和代谢方法的血管系统研究。

• 批准号: 01101013
• 负责人: EBASHI Setsuro
• 金额: --
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Special Project Research.
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01101013/
• 关键词: vasoactive peptides; endothel growth factor; culture of vascular system; bioactive lipides; Ca^<2+> in vascular system; intracellular Ca^<2+> concentration; regulatory proteins of smooth muscle

The research group has achieved a remarkable success in many respects of the field: 1) An enormous development has been attained in introducing molecular genetic approach to this field. This technique has become a common method for most of the members of this group. 2) A tremendous progress has been made in the studies on the interaction between endothel and smooth muscle. 3) Role of lipoproteins in pathogenesis of arterioscherosis were studied with special reference to macrophage. 4) Notable findings were obtained in the studies on structural proteins which regulate the contractile proteins of smooth Muscle. 5) The method of measuring intracellular Ca^<2+> concentrations in active state was markedly advanced. 6) Reexamination of various peptides affecting vascular system was carried out from the cardiovascular points of view. 7) Studies on the blood flow from the viewpoints of not only theology but also metabolism has provided a new scope as to the spasm and thrombosis in vascular system. 8) Various kinds of experimental models were explored, which will be a very useful tool for cardiovascular research.As a whole, a new concept "Endothel-smooth muscle coupling" has emerged as the next subject of research in this field.

本研究组在该领域的许多方面取得了显著的成就：1）在将分子遗传学方法引入该领域方面取得了巨大的发展。这一技术已成为该群体大多数成员的常用方法。2)内皮细胞与平滑肌相互作用的研究取得了巨大的进展。3)本文以巨噬细胞为研究对象，探讨脂蛋白在动脉硬化发病中的作用。4)在调节平滑肌收缩蛋白的结构蛋白的研究中获得了显著的发现。5)测定活性状态下细胞内Ca^<2+>浓度的方法有了显著的进步. 6)从心血管的角度重新审视了影响血管系统的各种肽。7)从神学和代谢的角度研究血流，为研究血管系统的痉挛和血栓形成提供了新的视野。8)各种实验模型的建立为心血管研究提供了一个非常有用的工具，“内皮-平滑肌偶联”的新概念已成为该领域的下一个研究课题。

项目成果

Ebashi, S.: "Development of Ca^<2+> concept in smooth muscle research" Frontiers in Smooth Muscle Research. 159-165 (1990)

Ebashi, S.：“平滑肌研究中 Ca^<2> 概念的发展”平滑肌研究前沿。

Ebashi,S.: "Development of Ca^<2+> concept in smooth muscle research" Frontiers in Smooth Muscle Research. 159-165 (1990)

Ebashi,S.：“平滑肌研究中 Ca^<2> 概念的发展”平滑肌研究前沿。

Yamano,T.,Nonaka,Y.,Okamoto,M.,Matsubara,T.,and Miura,R.: "Rate enhancement of the electron transfer of the adrenodoxin-adrenodoxin reductase system by dicarboxylic acids" Biochem.Biophys.Res.Commun.162. 168-174 (1989)

Yamano,T.、Nonaka,Y.、Okamoto,M.、Matsubara,T. 和 Miura,R.：“二羧酸增强肾上腺素还蛋白-肾上腺素还素还原酶系统的电子转移速率”Biochem.Biophys.Res。

Nakamura,S.,Mabeshima,Y.,Kawashima,M.,Nakamura,S.,Nonomura,Y., Fujii,Y.: "Isolation of the chick myosin aokali light chain gene expressed in embryonic gizzard muscle and transitional expression of the light chain gene family in vivo." J.Mol.Biol.204. 497-

Nakamura，S.，Mabeshima，Y.，Kawashima，M.，Nakamura，S.，Nonomura，Y.，Fujii，Y.：“在胚胎砂囊肌肉中表达的鸡肌球蛋白 aokali 轻链基因的分离和过渡表达

Horie,M.,Irisawa,H.: "Dual effects of intracellular magnesium on muscarinic potassium channel current in single guinea-pig atrial cells." J.Physiol.408. 313-332 (1989)

Horie,M.,Irisawa,H.：“细胞内镁对单个豚鼠心房细胞中毒蕈碱钾通道电流的双重影响。”