Collaborative survey and utilization of animal species and active substances useful for physiological research in China

我国生理学研究有用动物种类及活性物质的协同调查与利用

• 批准号: 62043080
• 负责人: EBASHI Setsuro
• 金额: $ 0.96万
• 依托单位: Institute of Physiological Sciences, Okazaki National Research Institutes
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62043080/
• 关键词: Scorpion; Scorpion Toxin; Na-channel; Ionic composition of haemolymph; Myelin; Fluorescent dye; クモ毒

We made a survey and a field research in Henan Province and in Takuramakan desert to find and collect scorpions and venomous spiders. Then, abdominal nerve fibers of them were processed for electron microscopy, blood samples were collected for assay, and electrophysiological studies were done on the nerve fibers. The venom of the scorpion and the spider were purified at Shanghai. Physiological and biochemical properties of the scorpion and scorpion toxin was studied as follows: The venom contained a neurotoxin that exerts effects rather selectively on insects. We purified this toxin and determined the amino acid sequence. The purified scorpion neurotoxin(BmK I) did not have any effect on electrophysiological properties of the scorpion nerve fiber. The ionic composition and the osmorality of the scorpion haemolymph was found to be very high for a terrestrial animal. The abdominal nerve cord did not have the giant axon but it contained a primitive type of myelin structure. The venom of the spider was collected and fractionated into 5 portions. They are stored in our laboratory together with the purified scorpion toxins and are prepared for further analyses.

为寻找和采集蝎子和毒蜘蛛，我们在河南省和塔克拉玛干沙漠进行了实地调查和研究。然后，对它们的腹部神经纤维进行电镜处理，采集血样进行分析，并对神经纤维进行电生理研究。蝎毒和蜘蛛毒是在上海提纯的。本文对蝎毒及其毒素的生理生化特性进行了研究，结果表明：蝎毒中含有一种对昆虫有选择性作用的神经毒素。我们纯化了该毒素并测定了其氨基酸序列。纯化的蝎神经毒素（BmK I）对蝎神经纤维的电生理特性没有任何影响。蝎子血淋巴的离子组成和渗透压被认为是非常高的陆生动物。腹神经索没有巨大的轴突，但它含有原始类型的髓鞘结构。收集蜘蛛的毒液并分成5份。它们与纯化的蝎子毒素一起储存在我们的实验室中，并准备用于进一步分析。