Usefulness of Dimethyl Sulfoxide (DMSO) for Protein

二甲基亚砜 (DMSO) 对蛋白质的用途

• 批准号: 01870016
• 负责人: EBASHI Setsuro
• 金额: $ 3.26万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01870016/
• 关键词: Dimethyl sulfoxide(DMSO); Dimethyl sulfoxide(DMSO) fractionation; Protein fractionation; Organic solvent fractionation; Ammonium sulfate fractionation; DMSO; アセトン分画

Usefulness of Dimethyl sulfoxide (DMSO) for Protein FractionationIt was incidentally found that DMSO is a useful material for protein fractionation.General properties of DMSO as the reagent for fractionation are not essentially different from other organic solvents such as ethanol and acetone. Its protein-deteriorating effect, however, is much less than these organic solvents at 0^ﾟC. If the experiments are carried out at a temperature lower than 10^ﾟC, DMSO can be used in a similar way to ammonium sulfate.On addition to water, DMSO produces a large mixing heat. If due caution is exercised in this respect, however, DMSO shows the same usefulness as that of ammonium sulfate. In some cases it proves more beneficial than the latter ; for instances, the protein fraction obtained by DMSO can be applied to electrophoresis and chromatography without eliminating this reagent. Sequential use of DMSO and ammonium sulfate sometimes gives rise to a sharp discrimination between two proteins that are not separable from each other by ordinary fractionation.Further details for the use of DMSO as the protein fractionating reagent were described and discussed.

二甲基亚砜（DMSO）在蛋白质分级分离中的应用人们偶然发现DMSO是一种有用的蛋白质分级分离材料，它作为分级分离试剂的一般性质与其它有机溶剂如乙醇和丙酮没有本质区别。然而，它对蛋白质的破坏作用在0 ℃时比这些有机溶剂小得多。如果实验是在低于10 ° C的温度下进行的，DMSO可以以类似于硫酸铵的方式使用。然而，如果在这方面谨慎行事，DMSO显示出与硫酸铵相同的有用性。在某些情况下，它被证明比后者更有益;例如，通过DMSO获得的蛋白质级分可以应用于电泳和色谱，而无需消除该试剂。DMSO和硫酸铵的顺序使用有时会引起两种蛋白质之间的明显区别，这两种蛋白质不能通过普通的分级分离彼此。

项目成果

江橋 節郎: "DMSO(dimethyl sufoxide)によるタンパク質分画法" 生化学. 63. (1991)

Setsuro Ebashi：“使用 DMSO（二甲基亚砜）的蛋白质分级分离方法”生物化学 63。（1991）

Eto, M., Suzuki, R., Morita, F., Kuwayama, H., Nishi, N., & Tokura, S.: "Roles of the amino acid side chains in the actin-binding S-site of myosin hevy chain" J. Biochem.108, No. 3. 499-504 (1990)

埃托，M.，铃木，R.，森田，F.，桑山，H.，西，N.，

Ebashi, S.: "Development of Ca^<2+> concept in smooth muscle research." Frontiers in smooth muscle research (eds. by N. Sperelakis and J. D. Wood) Alan R. Liss, Inc. New York. 159-165 (1990)

Ebashi, S.：“平滑肌研究中 Ca^<2> 概念的发展。”

Kuwayama,H.,Suzuki,M.,Koga,R.and Ebashi,S.: "Preparation of protein components exhibiting myosin light chain kinase activities from bovine aorta:Discrepancies between its enzyme activity and actomyosin activating effect" J.Biochem.104. 862-866 (1988)

Kuwayama,H.,Suzuki,M.,Koga,R.和Ebashi,S.：“从牛主动脉中制备表现出肌球蛋白轻链激酶活性的蛋白质成分：其酶活性与肌动球蛋白激活作用之间的差异”J.Biochem.104

Ebashi,S.and Kuwayama,H.: "Calcium regulation of smooth muscle contraction" In Calcium Signal and Cell Response(eds.by Yagi,k.and Miyazaki,T)Japan Sci.Soc.Press,Tokyo,Springer-Verlag,Berlin. 143-150 (1988)

Ebashi,S. 和 Kuwayama,H.：钙信号和细胞反应中的“平滑肌收缩的钙调节”（Yagi,k. 和 Miyazaki,T 编辑）Japan Sci.Soc.Press，东京，Springer-Verlag，