Usefulness of Dimethyl Sulfoxide (DMSO) for Protein

二甲基亚砜 (DMSO) 对蛋白质的用途

• 批准号: 01870016
• 负责人: EBASHI Setsuro
• 金额: $ 3.26万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01870016/
• 关键词: Dimethyl sulfoxide(DMSO); Dimethyl sulfoxide(DMSO) fractionation; Protein fractionation; Organic solvent fractionation; Ammonium sulfate fractionation; DMSO; アセトン分画

Usefulness of Dimethyl sulfoxide (DMSO) for Protein FractionationIt was incidentally found that DMSO is a useful material for protein fractionation.General properties of DMSO as the reagent for fractionation are not essentially different from other organic solvents such as ethanol and acetone. Its protein-deteriorating effect, however, is much less than these organic solvents at 0^ﾟC. If the experiments are carried out at a temperature lower than 10^ﾟC, DMSO can be used in a similar way to ammonium sulfate.On addition to water, DMSO produces a large mixing heat. If due caution is exercised in this respect, however, DMSO shows the same usefulness as that of ammonium sulfate. In some cases it proves more beneficial than the latter ; for instances, the protein fraction obtained by DMSO can be applied to electrophoresis and chromatography without eliminating this reagent. Sequential use of DMSO and ammonium sulfate sometimes gives rise to a sharp discrimination between two proteins that are not separable from each other by ordinary fractionation.Further details for the use of DMSO as the protein fractionating reagent were described and discussed.

偶然发现DMSO是蛋白质分馏的有用材料。二甲基磺代（DMSO）对蛋白质分馏的有用性。DMSO作为分级试剂的一般特性与其他有机溶液（如乙醇和丙酮）没有基本不同。然而，它的蛋白质偏置作用远低于0^ﾟC的这些有机溶液。如果在低于10^c的温度下进行实验，则可以以与硫酸铵相似的方式使用DMSO。在水中，DMSO会产生较大的混合热。但是，如果在这方面谨慎行事，DMSO显示出与硫酸铵相同的有用性。在某些情况下，它比后者更有益。对于情况下，通过DMSO获得的蛋白质分数可以应用于电泳和色谱法，而无需消除该试剂。 DMSO和硫酸铵的顺序使用有时会引起两种蛋白质之间的尖锐区分，两种蛋白质并非通过普通分馏。用于使用DMSO的细节，以描述并讨论蛋白质分级试剂。

项目成果

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Ebashi,S.（N.Sperelakis 和 J.D.Wood 编辑）：“平滑肌研究中 Ca^<2> 概念的发展。平滑肌研究的前沿”Alan R.Liss,Inc.纽约，159-165

Ebashi, S. and Kuwayama, H.: "Calcium regulation of smooth muscle contraction." Calcium Signal and Cell Response (eds. by Yagi, K. and Miyazaki, T.) Japan Sci. Soc. Press, Tokyo, Springer-Verlag, Berlin. 143-150 (1988)

Ebashi, S. 和 Kuwayama, H.：“平滑肌收缩的钙调节。”

Ebashi, S.: "Calcium Regulation of Smooth Muscle Contraction." Muscle Energetics (eds. by Paul, R. J., Elzinga, G. and Yamada, K.) Alan R. Liss, New York. 239-251 (1989)

Ebashi, S.：“平滑肌收缩的钙调节。”

Ebashi,S.: "Calcium Regulation of Smooth Muscle Contraction.In Muscle Energetics(eds.by Paul,P.L.,Elzinga,G.and Yamada,K.)" Alan R.Liss,New York, 239-251 (1989)

Ebashi,S.：“平滑肌收缩的钙调节。肌肉能量学（Paul,P.L.、Elzinga,G. 和 Yamada,K. 编辑）”Alan R.Liss，纽约，239-251 (1989)

Kuwayama,H.,Suzuki,M.,Koga,R.and Ebashi,S.: "Preparation of protein components exhibiting myosin light chain kinase activities from bovine aorta:Discrepancies between its enzyme activity and actomyosin activating effect" J.Biochem.104. 862-866 (1988)

Kuwayama,H.,Suzuki,M.,Koga,R.和Ebashi,S.：“从牛主动脉中制备表现出肌球蛋白轻链激酶活性的蛋白质成分：其酶活性与肌动球蛋白激活作用之间的差异”J.Biochem.104