Usefulness of Dimethyl Sulfoxide (DMSO) for Protein

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Usefulness of Dimethyl sulfoxide (DMSO) for Protein FractionationIt was incidentally found that DMSO is a useful material for protein fractionation.General properties of DMSO as the reagent for fractionation are not essentially different from other organic solvents such as ethanol and acetone. Its protein-deteriorating effect, however, is much less than these organic solvents at 0^ﾟC. If the experiments are carried out at a temperature lower than 10^ﾟC, DMSO can be used in a similar way to ammonium sulfate.On addition to water, DMSO produces a large mixing heat. If due caution is exercised in this respect, however, DMSO shows the same usefulness as that of ammonium sulfate. In some cases it proves more beneficial than the latter ; for instances, the protein fraction obtained by DMSO can be applied to electrophoresis and chromatography without eliminating this reagent. Sequential use of DMSO and ammonium sulfate sometimes gives rise to a sharp discrimination between two proteins that are not separable from each other by ordinary fractionation.Further details for the use of DMSO as the protein fractionating reagent were described and discussed.