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Transmembrane Signaling

跨膜信号传导

基本信息

批准号：
01045035
负责人：
EBASHI Setsuro
金额：
$ 5.44万
依托单位：
Okazaki National Research Institutes (National Institute for Physiological Sciences)
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

This project aimed at elucidation of signal transduction pathway from membrane receptors to physiological cellular responses.1. First Year (1989) : We studied signal transduction pathway in muscle cells.(1) Involvement of lyotonin in contraction of smooth muscles was studied.(2) Involvement of inositol metabolites in Ca transients in skeletal muscles was studied.(3) Fura-2 visualization of Ca transients in bull-frog skeletal muscles was done.The threshold for caffeine stimulation for Ca release was measured to be 1-2 mM.2. Second Year (1990) : We studied exocrine glands and anti-channel drugs.(1) In colonic goblet cells, okadaic acid blocked the late part of the secretory and Ca responses induced by muscarinic stimulation. Thus, phosphorylation of muscarinic receptors mediates desensitization of these cellular responses.(2) Exocytotic responses in salivary gland of tupai were clearly detected by DIC microscopy. Water secretion was visualized as periodical movements of the apical membra … More ne of acinar cells. Muscarinic stimulants and ATP were effective but BETA stimulants were not in inducing secretory responses in the sublingual gland.(3) Toosendainine was found to suppress the Ca channel activity in NG108 cells.(4) A neurotoxin that specifically blocks the Na channel in insects was purified from the scorpion venom.3. Third Year (1991) : Stimulation-secretion coupling in tupai salivary gland and glutamate responses in retinal horizontal cells were studied.(1) Either various activators of protein kinase C nor K-depolarization were not effective in inducing secretory response in the salivary gland. These suggested that transmembrane signaling in salivary gland is mediated by a 2nd messenger system other than A-kinase and C-kinase systems.(2) Kinate-induced currents were studied in horizontal cells. Dopamine modulated the kinate current in a dose-dependent manner. Haroperidol suppressed the effect of dopamine on the kinate current. These modulatory effects are thought to arise from alteration of cGMP messenger system. Less

本项目旨在阐明从膜受体到生理细胞反应的信号转导途径.第一年（1989年）：我们研究了肌肉细胞中的信号转导途径。(1)研究了Lyotonin在平滑肌收缩中的作用。(2)研究了肌醇代谢产物在骨骼肌钙瞬变中的作用。(3)Fura-2显示牛蛙骨骼肌中的钙瞬变，测量咖啡因刺激钙释放的阈值为1-2 mM。第二年（1990年）：我们研究了外分泌腺和反通道药物。(1)在结肠杯状细胞，冈田酸阻断毒蕈碱刺激诱导的分泌和钙反应的后期。因此，毒蕈碱受体的磷酸化介导这些细胞反应的脱敏。(2)DIC显微镜下可清楚地观察到吐排犬唾液腺的胞吐反应。水分泌表现为顶膜的周期性运动 ...更多信息腺泡细胞的一种。毒蕈碱刺激剂和ATP是有效的，但BETA刺激剂不能诱导舌下腺的分泌反应。(3)川楝素可抑制NG 108细胞钙通道活性。(4)从蝎毒中纯化出一种特异性阻断昆虫Na通道的神经毒素。第三年（1991年）：研究了图派唾液腺的刺激-分泌偶联和视网膜水平细胞的谷氨酸反应。(1)无论是各种蛋白激酶C的激活剂，也没有K-去极化诱导唾液腺分泌反应是有效的。提示唾液腺跨膜信号转导是由第二信使系统介导的，而不是由A激酶和C激酶系统介导的。(2)在水平细胞中研究了激动剂诱导的电流。多巴胺以剂量依赖性方式调制激动电流。Haroperidol抑制多巴胺对激动电流的作用。这些调节作用被认为是由cGMP信使系统的改变引起的。少