SP1 - Epigenetic acclimation to abiotic and biotic stress in clonal oaks.

SP1 - 克隆橡树对非生物和生物胁迫的表观遗传适应。

• 批准号: 520752203
• 负责人: Professorin Dr. Katrin Heer
• 金额: --
• 依托单位: Équipe E4E - Evolution dans les écosystèmes forestiers
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/520752203?language=en
• 关键词: SP1; Epigenetic; acclimation; abiotic; biotic

Climate change and global species loss are the major environmental threats to human well-being in the coming decades. However, despite more than 200 years of organized forestry and research, some fundamental knowledge is still missing about (i) the phenotypic plasticity of forest trees, (ii) about the interplay of trees with their microbial symbionts, and (iii) about how these interactions may facilitate acclimation (regulatory changes) and adaptation based on genetic changes of trees and/or their symbionts. In PhytOakmeter, we seek to understand patterns and mechanisms of acclimation and adaptation (A&A) in trees focusing on of a holobiont tree model. In Subproject 1 - Epigenetic acclimation to abiotic and biotic stress in clonal oaks - we investigate methylation changes and the activation of transposable elements (TEs) in the oak clone DF159 in response to drought and herbivory treatments. Specifically, we seek to understand the dynamics of methylation patterns and TE activation under subsequent stress events. The clone DF159 is particularly suited for such research since the absence of genetic variation allows targeting stochastic and environmentally induced epimutations. The three experimental platforms will allow us to investigate methylation and TEs activation under highly controlled to natural conditions. Ultimately, we seek to jointly analyze our data together with data on gene expression, metabolome changes, and physiological traits to understand how responses are coordinated across different layers of cellular organization. Moreover, to better understand the clonal oak system, we will investigate the effect of clonal propagation from lateral vs. apical buds as well as the crosstalk between scion and rootstock in grafted oak clones. Overall, SP1 seeks to enhance our understanding of how trees respond to stress at the level of the methylome and the activation of transposable elements (TEs), and how these changes impact the plant´s response to subsequent stresses.

气候变化和全球物种丧失是未来几十年对人类福祉的主要环境威胁。然而，尽管有200多年的有组织的林业和研究，一些基本的知识仍然缺乏（i）林木的表型可塑性，（ii）关于树木与其微生物共生体的相互作用，以及（iii）关于这些相互作用如何促进适应（调节变化）和适应基于树木和/或其共生体的遗传变化。在PhytOakmeter中，我们试图了解树木适应和适应（A&A）的模式和机制，重点是全生树模型。在子项目1 -表观遗传驯化克隆橡树的非生物和生物胁迫-我们调查甲基化的变化和激活的转座因子（TE）在橡树克隆DF 159响应干旱和草食动物治疗。具体来说，我们试图了解甲基化模式和TE激活在随后的压力事件下的动态。克隆DF 159特别适合于这种研究，因为不存在遗传变异允许靶向随机和环境诱导的表突变。这三个实验平台将使我们能够在高度受控的自然条件下研究甲基化和TE活化。最终，我们寻求联合分析我们的数据以及基因表达，代谢组变化和生理特征的数据，以了解反应如何在细胞组织的不同层之间协调。此外，为了更好地了解克隆橡树系统，我们将调查克隆繁殖的影响，从侧芽与顶芽，以及嫁接橡树无性系的接穗和砧木之间的串扰。总的来说，SP1旨在提高我们对树木如何在甲基化水平上应对压力和转座因子（TE）激活的理解，以及这些变化如何影响植物对后续压力的反应。