Coordination Funds

协调基金

• 批准号: 520845685
• 负责人: Professor Dr. Lars Opgenoorth
• 金额: --
• 依托单位: Arbeitsgruppe Pflanzenökologie und Geobotanik
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/520845685?language=en
• 关键词: Coordination; Funds

Climate change and global species loss are the major environmental threats to human well-being in the coming decades. However, despite more than 200 years of organized forestry and research, some fundamental knowledge is still missing about (i) the phenotypic plasticity of forest trees, (ii) about the interplay of trees with their microbial symbionts, and (iii) about how these interactions may facilitate acclimation (regulatory changes) and adaptation (A&A) based on genetic changes of trees and/or their symbionts. Therefore PhytOakmeter seeks to derive such patterns and mechanisms of acclimation and adaptation (A&A) of a holobiont tree model by setting up substantial experimental resources to develop a new tree model. In practice, we will establish (i) a tree model system with sufficient -omics resources to facilitate the investigation of A&A patterns from a holobiont perspective in forest trees, (ii) mesocosm experiments ranging from complete environmental and stress control to mesocosm experiments with partly natural environments, (iii) relate experimental findings mostly gained from saplings to observations on mature trees, and (iv) establish the monitoring of model trees under a broad range of natural conditions, including extreme sites. Specifically, we will mainly work on three experimental platforms with the clone DF159 to (i) perform controlled experiments in two Ecotrons that expose the holobiont to two moderate droughts (previous year and current year for longer-term, spring and summer for shorter term stress memory) and above- and below-ground herbivory, respectively; (ii) expose oak clonal saplings in the canopy of mature trees in comparison to resident canopy twigs and saplings at ground level; and (iii) assess clonal oak saplings released across Germany and Europe for analyzing A&A mechanisms under a wide range of environmental conditions. In Subproject 1 - Epigenetic acclimation to abiotic and biotic stress in clonal oaks - we will investigate methylation changes and Transposable Elements in relation to the drought and herbivory treatment of the three experimental Platforms.

气候变化和全球物种丧失是未来几十年人类福祉的主要环境威胁。然而，尽管有200多年的有组织的林业和研究，关于以下方面的一些基本知识仍然缺失：(i)森林树木的表型可塑性，（ii）树木与其微生物共生体的相互作用，以及（iii）这些相互作用如何促进基于树木和/或其共生体遗传变化的驯化（调节变化）和适应（A&A）。因此，PhytOakmeter试图通过建立大量的实验资源来开发新的树模型，从而获得全生物树模型的驯化和适应（A&A）模式和机制。在实践中，我们将建立(i)一个具有足够的组学资源的树木模型系统，以方便从整体生物学角度研究森林树木的A&A模式；（ii）从完全环境和胁迫控制到部分自然环境的中生态实验；（iii）将主要从树苗中获得的实验结果与成熟树木的观察相关联。（iv）在广泛的自然条件下，包括极端地点，建立对模型树的监测。具体而言，我们将主要利用DF159克隆在三个实验平台上进行(i)在两个Ecotrons中进行对照实验，分别将holobiont暴露于两次中度干旱（长期为上一年和今年，春季和夏季为短期应激记忆）和地上和地下草食；（ii）将成熟树冠下的橡树无性系树苗与常住树冠枝条和地面树苗进行比较；（iii）评估在德国和欧洲释放的无性系橡树树苗，以分析各种环境条件下的A&A机制。在子项目1 -克隆橡树对非生物和生物胁迫的表观遗传驯化中，我们将研究三个实验平台在干旱和草食处理下甲基化变化和转座因子的关系。