Planar Capillary Electrophoresis

平面毛细管电泳

• 批准号: 2247387
• 负责人: Robert Dunn
• 金额: $ 47.28万
• 依托单位: University of Kansas Center for Research Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2247387&HistoricalAwards=false
• 关键词: Planar; Capillary; Electrophoresis

With support from the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry (CHE) and co-funding from the Established Program to Stimulate Competitive Research (EPSCoR), Professor Robert Dunn and his group at the University of Kansas are developing innovative, cost-effective approaches for rapidly analyzing complex mixtures. Mixtures such as blood and urine contain thousands of molecules that reflect the normal functions taking place within the human body. Hidden within these complicated fluids can be molecular clues about diseases or disorders, that if detected early can aid in their treatment. Dr. Dunn and his group are developing new approaches to rapidly detect these molecules by separating mixtures through small capillaries that are as thin as a human hair. The thin wall of the capillary enables unique control over fundamental separation parameters which will be developed to enhance detection. The research plan combines fundamental studies with applied measurements and elements of engineering, thus exposing students to a highly interdisciplinary and collaborative research environment. Combined with a novel sample droplet delivery system, the fully integrated analysis platform has the potential to provide significant new capabilities for separations science. This research project will train undergraduate and graduate students in a highly collaborative and interdisciplinary environment, leading to enhanced opportunities for professional development.Planar capillary electrophoresis (PCE) uses a short length capillary to rapidly separate and detect species in complex mixtures. The PCE uses a 10 cm total length capillary (50 um i.d. x 80 um o.d.) with an ultra-thin capillary wall (15 um) that efficiently dissipates heat and mitigates the effects of Joule heating. The utiization of an ultra-thin wall provides particular opportunities for developing new approaches and probing fundamental processes. For example, it facilitates the use of an externally applied field to modify the zeta potential and control electroosmotic flow (EOF). This allows facile control of the EOF and leads to a significant increase in peak efficiency. The mechanism for the latter will be fully explored through experiment and simulation and optimized to improve performance. The thin capillaries are also highly flexible, enabling looped geometries that bring the separation path across the detection zone multiple times. This will be developed for kinetic studies of ligand-binding. Finally, the small capillary diameter will enable coupling with digital microfluidics (DMF) to develop fully integrated analysis and economical platforms that have great potential in terms of versatility.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学部（CHE）的化学测量和成像（CMI）计划的支持下，并在刺激竞争性研究（EPSCoR）的既定计划的共同资助下，堪萨斯大学的Robert Dunn教授及其团队正在开发快速分析复杂混合物的创新，具有成本效益的方法。 血液和尿液等混合物含有数千种分子，反映了人体内发生的正常功能。 隐藏在这些复杂液体中的可能是疾病或紊乱的分子线索，如果早期发现，可以帮助治疗。 Dunn博士和他的团队正在开发新的方法，通过像人的头发一样细的小毛细管分离混合物来快速检测这些分子。 毛细管的薄壁使得能够对基本分离参数进行独特的控制，这些参数将被开发以增强检测。 该研究计划将基础研究与应用测量和工程元素相结合，从而使学生接触到高度跨学科和协作的研究环境。 结合新颖的样品液滴输送系统，完全集成的分析平台有可能为分离科学提供重要的新功能。该研究项目将在高度合作和跨学科的环境中培养本科生和研究生，从而增强专业发展的机会。平面毛细管电泳（PCE）使用较短长度的毛细管快速分离和检测复杂混合物中的物种。 PCE使用总长度为10 cm的毛细管（内径为50 um）。x 80 um外径）具有超薄毛细管壁（15 um），可有效散热并减轻焦耳热效应。 超薄壁的利用为开发新方法和探索基本过程提供了特别的机会。 例如，它有助于使用外部施加的场来改变zeta电位和控制电渗流（electrosomial flow，简称electrosomial flow）。 这允许容易地控制功率，并导致峰值效率的显著增加。 后者的机制将通过实验和仿真充分探索，并优化，以提高性能。 薄的毛细管也是高度灵活的，能够实现环形几何形状，使分离路径多次穿过检测区。 这将被开发为配体结合的动力学研究。 最后，小的毛细管直径将能够与数字微流体（DMF）耦合，开发完全集成的分析和经济平台，在多功能性方面具有巨大潜力。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。