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Plasma Separation with Inorganic Membranes Using Bovine Blood

使用牛血通过无机膜分离血浆

基本信息

批准号：
60550672
负责人：
SAKAI Kiyotaka
金额：
$ 1.15万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60550672/
关键词：
Ceramic membrane Plasma separation Microfiltration Sieving coefficient Elution Regeneration Membrane structure 孔径分布

项目摘要

Techniques of membrane plasma separation consist of the microfiltration method in which a pressure-driven membrane process is used to separate plasma from blood in therapeutic and donor apheresis. The objective of the present study is to develop inorganic membranes for plasma separation.1. Elution Test : Elution tests using either pure water, 1N nitric acid, 500ppm sodium hypochlorite or bovine plasma were made to elucidate the elution of membrane constitutes from the sintered ceramic and porous glass membranes tested, based on the Standards for Approval of Hemodialyzers, the Ministry of Health and Welfare, Japan. Sintered ceramic membranes of aluminum oxide eluted fairly small amounts of membrane constituents. No elution of aluminum were found for porous glass membranes, while large amounts of boron and silicon were eluted with each test solution. After rinsing with nitric acid, boron and silicon were eluted in small amounts with pure water and in large amounts with bovine plasma. Surface modification is required to prevent the elution of boron and silicon from porous glass membranes.2. Filtration Test : Porous glass membranes were developed for plasma separation. Porous glass membranes over 4x <10^(-7)> m in pore diameter and over 40% in porosity were practically equal to polymer membranes in filtration characteristics. No hemolysis was observed for any porous glass membranes even at elevated transmembrane pressures. Rinsing with chemicals allowed complete and repeated regeneration of fouled membranes. This is a major advantage compared to polymer membranes. Maximal filtrate flux was obtained for membranes with a pore diameter of 1x <10^(-6)> m at a transmembrane pressure of 13.3 kPa and a wall shear rate of 2000 <s^(-1)> , which was about four times that of polymer membranes.

膜血浆分离技术包括微滤方法，其中压力驱动膜过程用于在治疗和供体单采中从血液中分离血浆。本研究的目的是开发用于血浆分离的无机膜.洗脱试验：根据日本厚生省血液透析器批准标准，使用纯水、1 N硝酸、500 ppm次氯酸钠或牛血浆进行洗脱试验，以阐明膜成分从受试烧结陶瓷和多孔玻璃膜中的洗脱。氧化铝烧结陶瓷膜洗脱了相当少量的膜成分。对于多孔玻璃膜，未发现铝洗脱，而每种测试溶液洗脱了大量的硼和硅。用硝酸冲洗后，用纯水洗脱少量硼和硅，用牛血浆洗脱大量硼和硅。为了防止硼和硅从多孔玻璃膜上洗脱，需要进行表面改性.过滤试验：开发了用于血浆分离的多孔玻璃膜。孔径大于4 × 10~（-7）m、孔隙率大于40%的多孔玻璃膜的过滤性能与聚合物膜相当。即使在升高的跨膜压力下，也未观察到任何多孔玻璃膜的溶血。用化学品冲洗允许完全和重复再生污染的膜。这是与聚合物膜相比的主要优点。在13.3 kPa的跨膜压力和2000 s ^（-1）的壁面剪切速率下，孔径为1 × 10 ^（-6）m的膜获得了最大的滤液通量，约为聚合物膜的4倍。