Ion Selective Transport Across Liquid Membrane Mimicking Renal Tubule Function

进一步改进人工肾血液透析治疗需要开发一种新的肾小管系统，该系统只能从血液中清除尿毒症毒素，或从流出的透析液中恢复必需离子和溶质并将其返回血液。本研究的目的是分离离子（Na^+, Cl^-）和尿素，并从流出的透析液中回收离子。利用电位差作为动力，采用离子选择性液膜分离离子（Na^+, Cl^-）和尿素，并通过透析浓缩离子。聚四氟乙烯膜，浸渍了有机溶液，其中形成反胶束或螯合剂溶解作为液体膜。使用反胶束液膜时，Na^+和Cl^-的传递速率高于尿素，并通过施加电位差而提高。采用螯合液膜，其膜稳定性高于反胶束液膜。另一方面，当不施加电位差时，Na^+和Cl^-通过液膜的速度与尿素一样快。然后利用反胶束液膜设计了模拟电渗析装置的三室间歇池。当外部两室之间施加电位差作为驱动力时，中间细胞的Na^+和Cl^-浓度增加，尿素浓度保持不变。因此，重复操作或连续操作，连接相同的电池，可以有效地分离离子（Na^+, Cl^-）和尿素，并浓缩离子（Na^+, Cl^-）。综上所述，利用反胶束液膜，三室批式细胞可以分离Na^+、Cl^-离子和尿素离子，并对NaCI和尿素溶液中的Na^+、Cl^-离子进行浓度测定，可以模拟肾小管的功能，从流出的透析液中回收必需离子和溶质返回血液。少

Further improvement of hemodialysis treatments with artificial kidney requires the development of a new renal tubule system that removes only uremic toxins from the blood, or recovers essential ions and solutes from outfiowing diatysate and returns them to the blood.The objective of the present study is to separate ions (Na^+, Cl^-) and urea and to recover the ions from the outfiowing dialysate. A potential difference was applied as a driving force using an ion selective liquid membrane to separate ions (Na^+, Cl^-) and urea and to concentrate the ions by dialysis. A PTFE membrane, impregnated with an organic solution in which reversed micelles were formed or chelate was dissolved was used as a liquid membrane. Using a reversed micelle liquid membrane, the transfer rate of Na^+ and Cl^- was higher than that of urea, and increased by applying the potential difference. Using a chelate liquid membrane, its membrane stability was higher than that of the reversed micelle liquid membrane. On … More the other hand, when no potential difference was applied, Na^+ and Cl^- permeated across the liquid membrane as fast as urea. Then a three chambers batch cell mimicking an electrodialysis apparatus was devised using the reversed micelle liquid membrane. When a potential difference was applied between outside two chambers as a driving force, Na^+ and Cl^- concentrations in the middle cell increased and urea concentration was kept to be constant. Therefore, repeated operation or continuous operation with the same cells connected makes it possible to separate ions (Na^+, Cl^-) and urea and concentrate ions (Na^+, Cl^-) efficiently.In conclusion, separation of ions (Na^+, Cl^-) and urea and concentration of ions (Na^+, Cl^-) in NaCI and urea solution with the three chambers batch cell are feasible using the reversed micelle liquid membrane, and the function of the renal tubule can be modeled and essential ions and solutes can be recovered from outflowing dialysate and returned to the blood. Less