Ion Selective Transport Across Liquid Membrane Mimicking Renal Tubule Function

模拟肾小管功能的离子选择性跨液膜运输

• 批准号: 09450307
• 负责人: SAKAI Kiyotaka
• 金额: $ 1.09万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450307/
• 关键词: Artificial kidney; Dialysis; Urea; Ion; Electrodialysis; Extraction; Surfactant; Chelate

Further improvement of hemodialysis treatments with artificial kidney requires the development of a new renal tubule system that removes only uremic toxins from the blood, or recovers essential ions and solutes from outfiowing diatysate and returns them to the blood.The objective of the present study is to separate ions (Na^+, Cl^-) and urea and to recover the ions from the outfiowing dialysate. A potential difference was applied as a driving force using an ion selective liquid membrane to separate ions (Na^+, Cl^-) and urea and to concentrate the ions by dialysis. A PTFE membrane, impregnated with an organic solution in which reversed micelles were formed or chelate was dissolved was used as a liquid membrane. Using a reversed micelle liquid membrane, the transfer rate of Na^+ and Cl^- was higher than that of urea, and increased by applying the potential difference. Using a chelate liquid membrane, its membrane stability was higher than that of the reversed micelle liquid membrane. On … More the other hand, when no potential difference was applied, Na^+ and Cl^- permeated across the liquid membrane as fast as urea. Then a three chambers batch cell mimicking an electrodialysis apparatus was devised using the reversed micelle liquid membrane. When a potential difference was applied between outside two chambers as a driving force, Na^+ and Cl^- concentrations in the middle cell increased and urea concentration was kept to be constant. Therefore, repeated operation or continuous operation with the same cells connected makes it possible to separate ions (Na^+, Cl^-) and urea and concentrate ions (Na^+, Cl^-) efficiently.In conclusion, separation of ions (Na^+, Cl^-) and urea and concentration of ions (Na^+, Cl^-) in NaCI and urea solution with the three chambers batch cell are feasible using the reversed micelle liquid membrane, and the function of the renal tubule can be modeled and essential ions and solutes can be recovered from outflowing dialysate and returned to the blood. Less

为了进一步改善人工肾血液透析治疗，需要开发一种新的肾小管系统，该系统仅从血液中去除尿毒症毒素，或从流出的透析液中回收必需的离子和溶质并将其返回血液。使用离子选择性液膜，以电势差作为驱动力，分离离子（Na^+，Cl^-）和尿素，并通过透析浓缩离子。将用其中形成了反胶束或溶解了螯合物的有机溶液浸渍的PTFE膜用作液膜。在反胶束液膜中，Na^+和Cl^-的迁移速率比尿素的迁移速率高，施加电位差后，Na^+和Cl^-的迁移速率增加。采用螯合液膜，其膜稳定性高于反胶束液膜。对 ...更多信息 另一方面，当不施加电位差时，Na^+和Cl^-的渗透速度与尿素一样快。然后利用反胶束液膜设计了一个仿电渗析装置的三室间歇电解槽。当在两个室之间施加电势差作为驱动力时，中间室中的Na^+和Cl^-浓度增加，而尿素浓度保持恒定。因此，重复操作或连续操作相同的细胞连接，使得分离离子成为可能（Na^+，Cl^-）和尿素以及浓缩离子（Na^+，Cl^-）的分离。总之，（Na^+，Cl^-）和尿素以及离子浓度采用反胶束液膜技术，用三室间歇式电解槽测定NaCl和尿素溶液中的Na^+，Cl^-，并且可以模拟肾小管的功能，并且可以从流出的透析液中回收必需的离子和溶质并返回到血液中。少