Detection and quantitation of rice dwarf virus-related nucleic acid in infected cells.

感染细胞中水稻矮缩病毒相关核酸的检测和定量。

• 批准号: 60560041
• 负责人: UYEDA Ichiro
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560041/
• 关键词: rice dwarf virus; cDNA cloning; 塩基配列

Rice dwarf virus (RDV) belongs to plant reovirus subgroup 1 and its genome consists of 12 segments of dsRNA. Gene segments 9 and 10 were cloned into Pst1 site of E. coli plasmid pBR 322 after homopolymeric tailing. cDNA inserts with apparent lengths of about 1300-1400 nucleotides were obtained for both segments 9 and 10. dscDNA of segment 10 was also made from viral transcript synthesized in vitro by a method of Gublar and Hoffman (1983). One clone of about 1,000 nucleotides in length was found to contain complete 5'end of the transcripts by primer extension and dideoxy sequencing analyses of both transcripts and the cDNA clone. Complete nucleotide sequence of gene segment 10 was determined.

水稻矮缩病毒（Rice dwarf virus，RDV）属于植物呼肠孤病毒亚组1，其基因组由12个dsRNA片段组成。将基因片段9和10克隆到E. coli质粒pBR 322。获得了片段9和10的表观长度约为1300-1400个核苷酸的cDNA插入片段。片段10的dscDNA也是通过Gublar和霍夫曼（1983）的方法从体外合成的病毒转录物制备的。通过引物延伸和转录物和cDNA克隆的双脱氧测序分析，发现长度约1，000个核苷酸的一个克隆含有完整的转录物5 '末端。测定了基因片段10的全核苷酸序列。

项目成果

Uyeda I., Matsumura, T., Sano, T., Ohshima, K., and Shikata, E.: "Molecular cloning and nucleotide sequence of ricedwarf virus S 10 RNA"

Uyeda I.、Matsumura, T.、Sano, T.、Ohshima, K. 和 Shikata, E.：“稻矮病毒 S 10 RNA 的分子克隆和核苷酸序列”