感染细胞中水稻矮缩病毒相关核酸的检测和定量。-猫眼课题宝

感染细胞中水稻矮缩病毒相关核酸的检测和定量。

批准号：

60560041

负责人：

UYEDA Ichiro

金额：

$1.28万

依托单位：

Hokkaido University

依托单位国家：

日本

项目类别：

Grant-in-Aid for General Scientific Research (C)

财政年份：

1985

资助国家：

日本

项目状态：

已结题

起止时间：

1985 至 1986

来源：

https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560041/

关键词：

rice dwarf virus cDNA cloning 塩基配列

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中文摘要

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英文摘要

Rice dwarf virus (RDV) belongs to plant reovirus subgroup 1 and its genome consists of 12 segments of dsRNA. Gene segments 9 and 10 were cloned into Pst1 site of E. coli plasmid pBR 322 after homopolymeric tailing. cDNA inserts with apparent lengths of about 1300-1400 nucleotides were obtained for both segments 9 and 10. dscDNA of segment 10 was also made from viral transcript synthesized in vitro by a method of Gublar and Hoffman (1983). One clone of about 1,000 nucleotides in length was found to contain complete 5'end of the transcripts by primer extension and dideoxy sequencing analyses of both transcripts and the cDNA clone. Complete nucleotide sequence of gene segment 10 was determined.

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Uyeda I., Matsumura, T., Sano, T., Ohshima, K., and Shikata, E.: "Molecular cloning and nucleotide sequence of ricedwarf virus S 10 RNA"

Uyeda I.、Matsumura, T.、Sano, T.、Ohshima, K. 和 Shikata, E.：“稻矮病毒 S 10 RNA 的分子克隆和核苷酸序列”

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