Molecular Mechanisms of Plant-Pathogenic Microbe Interaction-Toward Production of Disease Resistant Plants-

主要研究内容如下：1)通过信号转导参与各种植物防御反应的分子机制；2)病原微生物与寄主因子之间的相互作用，参与致病和寄主抗性机制。在研究期间，组织了研究会议、青年科学家研讨会和研讨会，出版了16份通讯。主要结果总结如下：1)发现病毒RNAi抑制活性与病毒基因组复制密切相关。这项研究开创了RNA沉默的一个新方面。2)植物的过敏性细胞死亡被发现是由遗传程序决定的，并提出了动植物之间执行细胞死亡的共同机制。3)在对病毒感染的防御反应中，SA和JA信号转导之间存在串扰。4)获得了抗疫霉菌的转基因马铃薯植株。这种防御反应被设计成只在病原体攻击植物时表达，而不是在伤口形成时表达。5)细胞壁被证明是第一个触发防御反应的因素，并分离出了参与其中的关键因素。6)CaN通道在系统获得性抗性中起关键作用。7)从分子水平上阐明了寄主特异性毒素在敏感寄主中的作用机制之一。

Studies are undertaken to elucidate the following ; 1) molecular mechanisms involved in various plant defense responses through the signal transduction, 2) interactions between pathogenic microbes and host factors involved in pathogenicity and host resistance mechanisms. During the studies, research meetings, seminars for the young scientists and symposia were organized and 16 newsletters have been published.Major results are summarized as below : 1) It was found that viral RNAi suppressor activity is closely linked with the virus genome replication. The study pioneered a new aspect of RNA silencing. 2) Hypersensitive cell death of plants was found to be genetically programmed and proposed there are common mechanisms among animals and plants that execute cell death. 3) Cross talk between SA and JA signal transductions are shown during defense response to viral infection. 4) Transgenic potato plant that is resistant to P. infestance was generated. The defense response is engineered to express only when pathogen attacks the plants but not when wound is made. 5) Cell wall was proved to be the first to trigger defense response and critical factors involved have been isolated. 6) Can-channel was shown to play a critical role in systemic acquired resistance. 7) One of molecular mechanisms how a host specific toxin acts in a susceptible host is elucidated at a molecular level.