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Protection from insect-borne plant viruses of rice and potato by transgenic plants.

通过转基因植物保护水稻和马铃薯免受虫媒植物病毒的侵害。

基本信息

批准号：
06556008
负责人：
UYEDA Ichiro
金额：
$ 4.8万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06556008/
关键词：
viral resistance gene potato rice transformation 抗体ウイルス遺伝子

项目摘要

Rice, tobacco, and potato plants were transformed by cDNA to viral genes of insect-borne viruses ; rice dwarf virus, potato leafroll virus, and potato virus Y.Tobacco plants were also transformed with the antibody gene of potato virus Y.And their resistance to viral infection was evaluated.1. Rice plants were transfomred with a coat protein gene of rice dwarf and rice ragged stunt viruses and the plants were inoculated with the viruses. None of the inoculated plants showed resistance and restriction of virual multiplication was not observed as assayd by ELISA.2. Potato plants were transformed with a coat protein and polymerase genes of potato leafroll virus and the plants were inoculated with the virus. Infection rate of the transgenic plants were the same as that of non-transgenic plants, but some restricted the virus multiplication.3. Tobacco plants were transformed with an antibody gene of potato virus Y and the plants were inoculated with the virus. None of the inoculated plants showed resistance to viral infecction.4. Tobacco plants were transformed with a coat protein gene of potato virus Y and the plants were inoculated with the virus. None of the inoculated plants showed resistance to viral infection.5. Based on above results, it was found that pathogen-derived resistance was difficult to acheive. And it was concluded search for host genes for resistance should be conducted in furture.

利用基因工程技术，将水稻、烟草和马铃薯分别转化为虫媒病毒、水稻矮缩病毒、马铃薯卷叶病毒和马铃薯Y病毒，并将马铃薯Y病毒抗体基因导入烟草植株，评价其对病毒侵染的抗性。将水稻矮缩病毒外壳蛋白基因和水稻矮化病毒外壳蛋白基因导入水稻植株，接种病毒。接种植株均未表现出抗性，ELISA检测未发现病毒增殖受到限制。将马铃薯卷叶病毒外壳蛋白和聚合酶基因分别转化马铃薯植株，并接种病毒。转基因植株的侵染率与非转基因植株相当，但有些植株的侵染率限制了病毒的增殖。将马铃薯Y病毒抗体基因导入烟草植株，并接种该病毒。接种植株均未表现出对病毒侵染的抗性。将马铃薯Y病毒外壳蛋白基因转化烟草植株，并接种该病毒。接种植株均未表现出对病毒侵染的抗性。以上结果表明，病原菌来源的抗性很难获得。因此，抗病寄主基因的寻找是今后的工作重点。