Molecular mechanism of viral pathogenicity and of viral mutplisation.

病毒致病性和病毒增殖的分子机制。

• 批准号: 12052201
• 负责人: UYEDA Ichiro
• 金额: $ 65.47万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12052201/
• 关键词: Plant virus; Pathogenicity; Multiplication; Host-virus Interactions; 相互作用; 植物ウイス; 宿主因子; Potyvirus; Cucumovirus; Bromovirus

HC-Pro gene of clover yellow vein virus was proven to play a crucial role in inducing necrosis in legume plants. Using an infectious cDNA to the virus, many mutants were generated and inoculated to plants. And several point mutations were identified controlling necrosis induction.We analyzed the functions of the 2b proteins of cucumoviruses in the viral symptom induction. The 2b protein of Tomato aspermy virus was found to be involved in the systemic movement in tobacco. The 2b protein of cucumber mosaic virus soybean strain induces seed coat mottling of soybean by suppressing RNA silencing of the chalcone synthase genes.The Arabidopsis CUM1 and CUM2 genes were cloned that are involved in cucumber mosaic virus multiplication. An in vitro assay system for the Tm-1 activity in tomato that inhibits the tomato mosaic virus RNA replication was also established.To analyze movement and adaptation mechanisms of bromoviruses, functional domains of the movement protein (MP) and coat protein of Brome mosaic virus (BMV) were mapped and a BMV MP-interacting host plant protein was identified and shown to be involved in cell-to-cell movement of BMV.

已证实三叶草黄脉病毒的HC-Pro基因在豆科植物的致死中起关键作用。利用病毒的感染性cDNA，产生了许多突变体并接种到植物上。我们分析了黄瓜病毒2b蛋白在病毒症状诱导中的作用。番茄无精症病毒的2b蛋白参与了烟草的系统运动。黄瓜花叶病毒大豆株系的2b蛋白通过抑制查尔酮合成酶基因的RNA沉默来诱导大豆种皮斑驳。为了分析雀麦花叶病毒(BMV)运动蛋白(MP)和外壳蛋白(CP)的功能域，确定了与BMV运动蛋白(MP)和外壳蛋白(CP)相互作用的寄主植物蛋白，并证明其参与了BMV的细胞间运动。

项目成果

Masuta, C.: "Development of clover yellow vein virus as an efficient, stable gene expression system for legume species."Plant Journal. 23. 539-546 (2000)

Masuta, C.：“将三叶草黄脉病毒开发为豆科植物高效、稳定的基因表达系统。”《植物杂志》。

Selective involvement of members of the eukaryotic initiation factor 4E family in the infection of Arabidopsis thaliana by potyviruses

• DOI: 10.1016/j.febslet.2004.12.086
• 发表时间: 2005-02
• 期刊: FEBS Letters
• 影响因子: 3.5
• 作者: Masanao Sato;K. Nakahara;Motoyasu Yoshii;M. Ishikawa;I. Uyeda

The Arabidopsis Cucumovirus Multiplication 1 and 2 Loci Encode Translation Initiation Factors 4E and 4G

• DOI: 10.1128/jvi.78.12.6102-6111.2004
• 发表时间: 2004-06
• 期刊: Journal of Virology
• 影响因子: 5.4
• 作者: Motoyasu Yoshii;M. Nishikiori;K. Tomita;Norimichi Yoshioka;R. Kozuka;S. Naito;M. Ishikawa

Mise, K.: "Use of Spring beauty latent virus to identify compatible interactions between bromovirus components required for virus infection."J.Gen.Virol.. 84. 1367-1375 (2003)

Mise, K.：“使用 Spring Beauty 潜伏病毒来识别病毒感染所需的溴病毒成分之间的兼容相互作用。”J.Gen.Virol.. 84. 1367-1375 (2003)

The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement

雀麦花叶病毒运动蛋白的C末端控制着细胞间运动对外壳蛋白的需求，在长距离运动中发挥作用

• 发表时间: 2004
• 期刊: Journal of General Virology 85
• 作者: Takeda;A.
• 通讯作者: A.