Studies on translocation of plant hormones in vascular tissue.

植物激素在维管组织中易位的研究。

• 批准号: 60560128
• 负责人: MUROFUSHI Noboru
• 金额: $ 0.96万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560128/
• 关键词: Plant hormone; Phloem; Vascular tissue; Gibberellin; Abscisic acid; イネ

It is important to establish very sensitive and reliable analysis methods for the investigation of plant hormones in vascular tissue of higher plants. For this purpose, several deuterium-labeled gibberellins, which are used as internal standards of GC/SIM analysis, were prepared by chemical or partly biological conversion. The simple method to introduce tritium into gibberellins was also establised. The translocation of plant hormones through vascular tissue was investigated by using phloem sap of rice obtained by laser-insect method as well as using honeydew excreted by brown planthopper. Gibberellin A_1(GA_1), GA_4, GA_<19>, GA_<20>, and GA_<29> were identified in honeydew by GC/SIM and the changes of their levels in honeydew at several growth stages of rice were observed. GA_<19> was the major gibberellin and its level increased at the stage of maximum tillering stage and then decreased at panicle initiation-heading stages. The levels of other gibberellins were low and there was no significant change of level.Abscisic scid(ABA) in honeydew was determined by gas-liquid chromatography using a electron capture detector. The level of ABA increased from 3rd leaf leaf stage, reached the maximum at maximum tillering stage and decreased at panicle initiation stage. ABA was identified by gas-liquid chromatography in the phloem sap obtained by laser-insect method. Hawever, gibberellins could not be identified in phloem sap due to their very low levels. To clarify the relationship between plant hormones in phloem sap and those in honeydew, the solution containing sucrose and gibberellins or ABA was fed to brown planthopper, and excreted honeydew was analyzed. Although there was not so large difference between gibberellins in sample solution and honeydew, clear conclusion on this problem has not been obtained.

建立灵敏可靠的分析方法对研究植物维管组织中植物激素的含量具有重要意义。为此目的，几个氘标记的赤霉素，这是用来作为GC/SIM分析的内标物，通过化学或部分生物转化制备。建立了将氚导入赤霉素的简便方法。利用激光-昆虫法获得的水稻韧皮部汁液和褐飞虱分泌的蜜露研究了植物激素通过维管组织的转运。采用气相色谱-离子选择法（GC/SIM）对水稻蜜露中的赤霉素A_1（GA_1）、GA_4、GA_4、GA_6、<19>GA_7<20>、GA_8进行了定性<29>分析，并观察了不同生育期蜜露中GA_1、GA_4、GA_8、GA_9的含量变化。GA_2<19>是主要的赤霉素，在最大分蘖期含量增加，在幼穗分化-抽穗期含量下降。其他赤霉素含量较低，且变化不显著。采用气液色谱-电子捕获检测器测定蜜露中脱落酸（阿坝）。阿坝含量从三叶期开始上升，在最大分蘖期达到最大值，在幼穗分化期下降。采用气液色谱法对激光-昆虫法提取的韧皮部汁液中的阿坝进行了鉴定。然而，赤霉素在韧皮部汁液中的含量很低，无法鉴定。为明确韧皮部汁液中植物激素与蜜露中植物激素的关系，以褐飞虱为试材，用赤霉素或脱落酸（阿坝混合溶液饲喂褐飞虱，并对褐飞虱分泌的蜜露进行分析。虽然样品溶液和蜜露中赤霉素的含量差异不大，但对这一问题还没有明确的结论。