Research on the substrate specificity of plant P450 and its regulatory function in plant metabolism.

植物P450的底物特异性及其在植物代谢中的调控功能研究。

• 批准号: 13660110
• 负责人: MUROFUSHI Noboru
• 金额: $ 0.77万
• 依托单位: Akita Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660110/
• 关键词: P450; Plant; Phytoalexin; Farnesol; Germacrene; Eudenmane; 植物二次代謝化合物; 植物培養細胞

The plant P450s are the group of oxidative enzymes in biosynthesis of the small molecule biologically active compounds in plant metabolism, and have important roles on the induction of those biological activities. Although the biologically active compounds have a diversity of chemical structures, the oxygen functionality in the structures is almost essential for the activity. Thus, the mechanisms to introduce those oxygens in the compounds are of quite interest to understand plant metabolism. For example, the phytoalexin, well-known antimicrobial compounds produced in the stressed plants, have a number of skeletons such as spirovetivanes and eremophilanes in Solanaceae species. The hydrocarbon skeleton itself does not show any remarkable activities, but once oxidized by P450 enzymes, the compounds become active. Deuterium labeled farnesol was synthesized and fed into green pepper seedlings, and the incorporation of the labeled precursor into the metabolites was confirmed. Furthermore, germacrene and spirovetivane hydrocarbon skeletons were synthesized in the way that deuterium or carbon-13 isotopes would be easily introduced. This research established the tools for metabolic experiments to explore the roles of these P450 enzymes.

植物P450是植物代谢过程中合成小分子生物活性化合物的一组氧化酶，在这些生物活性的诱导中起着重要作用。虽然具有生物活性的化合物具有多种化学结构，但结构中的氧官能团几乎是活性所必需的。因此，在化合物中引入这些氧素的机制对于了解植物的新陈代谢非常有意义。例如，植物抗菌素是在逆境植物中产生的众所周知的抗菌化合物，在茄科植物中具有许多骨架，如螺环烯和环烯丙烷。碳氢骨架本身没有表现出任何显著的活性，但一旦被P450酶氧化，化合物就会变得活跃。合成了标记的法尼醇，并将其饲喂到青椒幼苗中，证实了标记的前体在代谢产物中的掺入。此外，杰马林和螺环戊烷碳氢骨架的合成方法很容易引入氚或碳-13同位素。这项研究建立了代谢实验的工具，以探索这些P450酶的作用。