Research on the role and function of plant ingredients for the lipid and sugar metabolism in human.

植物成分对人体脂质和糖代谢的作用和功能研究。

• 批准号: 16580089
• 负责人: MUROFUSHI Noboru
• 金额: $ 2.43万
• 依托单位: Akita Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580089/
• 关键词: lipid; Eucommia ulmoides Oliver; plant; metabolism; 3T3-L1; obesity; vegetable; crabapple

The extract prepared from vegetables, crabapples, and the roasted Eucommia ulmoides Oliver leaves, Du-zhong tea, were examined to explore their effects on the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. More than 40 kinds of vegetable extracts were prepared and examined. Among them, several kinds showed the enhancement of lipid accumulation while another several kinds inhibited the accumulation. The active components were now under investigation. More than 40 kinds of crabapple extracts were examined for 3T3-L1 lipid accumulation and most of them inhibited its accumulation. The apple polyphenols were partially responsible for the activity. The boiling water extract of Du-zhong tea inhibited lipid accumulation in 3T3-L1. The HPLC analysis of the extract identified catechin, protocatechuic acid, pyrogallol, and chlorogenic acid. The activity guided separation of Du-zhong tea lead the isolation of 5-hydroxymethyl-2-furaldehyde (HMF). HMF inhibited lipid accumulation at the concentration of 100 μM. Upon feeding Du-zhong tea powder to chicken, the lipid metabolism was influenced by the activation of PPARα.

以小鼠3 T3-L1前脂肪细胞为研究对象，研究了山楂、杜仲炒奥利弗叶提取物对小鼠3 T3-L1前脂肪细胞分化的影响。制备并检测了40多种蔬菜提取物。其中，有几种植物表现出促进脂质积累的作用，而另几种则表现出抑制脂质积累的作用。目前正在对活性成分进行调查。对40余种海棠提取物进行了3 T3-L1脂质积累试验，多数提取物对3 T3-L1脂质积累有抑制作用。苹果多酚对该活性起部分作用。杜仲茶沸水提取物抑制3 T3-L1细胞的脂质积累。提取物的HPLC分析鉴定了儿茶素、原儿茶酸、连苯三酚和绿原酸。对杜仲茶进行活性导向分离，分离得到5-羟甲基-2-糠醛（HMF）。HMF在100 μM浓度下抑制脂质蓄积。杜仲茶粉对鸡的脂质代谢有一定的影响。

项目成果

The effect of vegetable extracts on the lipid accumulation in 3T3-L1 cells. (in Japanaese)

蔬菜提取物对 3T3-L1 细胞脂质积累的影响。

• 发表时间: 2005
• 期刊: Shokuhinkogyo(published by Kohrin) 48(8)
• 作者: Eriko Matsuda;Yuko Yoshizawa;Yuki Yokosawa;Naomi Watanabe;Satoru Kawaii;Noboru Murofushi;吉澤 結子;Yuko Yoshizawa
• 通讯作者: Yuko Yoshizawa

Effects of Eucommia ulmoides Oliver leaf extract on 3T3-L1 differentiation into adipocytes

• DOI: 10.1007/s11418-005-0026-y
• 发表时间: 2006-02
• 期刊: Journal of Natural Medicines
• 影响因子: 3.3
• 作者: Eriko Matsuda;Y. Yoshizawa;Yukihito Yokosawa;N. Watanabe;S. Kawaii;N. Murofushi

脂肪蓄積に及ぼす野菜の影響

蔬菜对脂肪堆积的影响

• 发表时间: 2005
• 期刊: 食品工業 48(8)
• 作者: Eriko Matsuda;Yuko Yoshizawa;Yuki Yokosawa;Naomi Watanabe;Satoru Kawaii;Noboru Murofushi;吉澤 結子
• 通讯作者: 吉澤 結子