Development of a freeze-drying instrument for plant tissues and its application to immunohistochemical and immunocytochemical analysis.

植物组织冻干仪的研制及其在免疫组织化学和免疫细胞化学分析中的应用。

• 批准号: 07558214
• 负责人: MUROFUSHI Noboru
• 金额: $ 0.96万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558214/
• 关键词: Immunocytochemistry; Immunohistochemistry; Plant hormones; gibberellins; freeze-drying instrument; gas fixation; イムノヒストケミストリー; 超低温凍結乾燥・固定・包埋

A freeze-drying instrument for immunohistochemical and immunocytochemical analysis of plant tissues was built by modifying an instrument which had been developed by Akahori for the immunocytochemical analysis of animal tissues. The following points were improved in the new instruments. (1) transfer system of samples frozen in liquid nitrogen (2) gas transfer system for fixation (3) resin transfer system for embedding. The temperature program optimizing freeze-drying of plant tissues is still under investigation. The optimum condition needs to be modified depending on tissues. This instrument was applied to the immunohistochemical analysis of gibberellins in developing seeds of Pharbitis nil, and enabled to visualized GAs localized on the starch grains in the seeds 9 days after anthesis.

通过对Akahori开发的用于动物组织免疫细胞化学分析的仪器进行修改，构建了用于植物组织免疫组织化学和免疫细胞化学分析的冷冻干燥仪器。新文书在以下几点上有所改进。(1)液氮冷冻样品转移系统（2）固定用气体转移系统（3）包埋用树脂转移系统。优化植物组织冷冻干燥的温度程序仍在研究中。最佳条件需要根据组织进行修改。该仪器被应用到生长中的牵牛种子中赤霉素的免疫组织化学分析，并使可视化的GAs定位于开花后9天的种子中的淀粉粒。