Regulation of glucose metabolism in chicken kidney

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The regulation of glucose metabolism was studied in chicken kidney perfused with either an arterial system or a portal system.A. Provided that kidneys were perfused with oxygenated buffer solution at a flow rate of 5 ml/min per g of kidney in both perfusion system, oxygenation of the kidney was achieved.B. Pyruvate, an oxidized substrate, found to be a much better gluconeogenic precursor than lactate in the perfused chicken kidney.C. Quinolinate, an inhibitor of cytosolic phosphoenolpyruvate carboxykinase, inhibited gluconeogenesis from lactate and pyruvate but not from succinate, malate and <alpha> -ketoglutarate.D. Chicken renal gluconeogenesis occurs in the cortex and glycolysis occurs in medullary structures. Furthermore, succinate, transported by a <Na^+> -dependent system and monocarboxylic acids transported by a <Na^+> -independent system through the basolateral side of the kidney are good substrates for gluconeogenesis.