Regulation of glucose metabolism in chicken kidney

鸡肾糖代谢的调控

• 批准号: 60560323
• 负责人: SUGANO Tsukasa
• 金额: $ 0.96万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60560323/
• 关键词: Chicken; Kidney; Liver; Perfusion; Gluconeogenesis; 糖分解; 代謝

The regulation of glucose metabolism was studied in chicken kidney perfused with either an arterial system or a portal system.A. Provided that kidneys were perfused with oxygenated buffer solution at a flow rate of 5 ml/min per g of kidney in both perfusion system, oxygenation of the kidney was achieved.B. Pyruvate, an oxidized substrate, found to be a much better gluconeogenic precursor than lactate in the perfused chicken kidney.C. Quinolinate, an inhibitor of cytosolic phosphoenolpyruvate carboxykinase, inhibited gluconeogenesis from lactate and pyruvate but not from succinate, malate and <alpha> -ketoglutarate.D. Chicken renal gluconeogenesis occurs in the cortex and glycolysis occurs in medullary structures. Furthermore, succinate, transported by a <Na^+> -dependent system and monocarboxylic acids transported by a <Na^+> -independent system through the basolateral side of the kidney are good substrates for gluconeogenesis.

本文研究了鸡肾动脉系统和门静脉系统对葡萄糖代谢的调节。如果在两个灌注系统中用充氧缓冲液以每克肾脏5 ml/min的流速灌注肾脏，则肾脏的充氧被阻断。丙酮酸是一种氧化底物，在灌流的鸡肾中，丙酮酸是一种比乳酸更好的促血管生成前体。细胞质磷酸烯醇丙酮酸羧激酶抑制剂喹啉酸抑制乳酸和丙酮酸的代谢，但不抑制琥珀酸、苹果酸和<alpha>β-酮戊二酸的代谢。鸡肾皮质中发生糖酵解，髓质结构中发生糖酵解。此外，通过依赖Na^+的系统转运的琥珀酸和通过不依赖Na^+的系统转运的一元羧酸通过肾脏的基底外侧是胚胎发生的良好底物。

项目成果

KATSUMI Yorita, TETSUO Yamano, KAZUHIKO Ikeda, TAKASHI Kobayashi and TSUKASA Sugano: "Distribution of glycolysis and gluconeogenesis in perfused chciken kidney." Am. J. Physiol.

KATSUMI Yorita、TETSUO Yamano、KAZUHIKO Ikeda、TAKASHI Kobayashi 和 TSUKASA Sugano：“灌注鸡肾中糖酵解和糖异生的分布。”

HIROAKI Niwa, TETSUO Yamano, TSUKASA Sugano, and Harris R. A.: "Hormonal effects and the cocntrol of gluconeogenesis from sorbitol, xylitol and glycerol in perfused chicken liver." Comp. Biochem. Physiol.85B. 739-745 (1986)

HIROAKI Niwa、TETSUO Yamano、TSUKASA Sugano 和 Harris R. A.：“灌注鸡肝中山梨醇、木糖醇和甘油的激素效应和糖异生作用的控制。”

NIWA Hiroyuki;YAMANO Tetsuo;SUGANO Tsukasa;HARRIS R.A.: Comp.Biochem.Physiol.85B. (1986)

NIWA Hiroyuki；YAMANO Tetsuo；SUGANO Tsukasa；HARRIS R.A.：Comp.Biochem.Physiol.85B。

YORITA Katsumi;YAMANO Tetsuo;IKEDA Kazuhiko;KOBAYASH Takashi;SUGANO Tsukasa: Am.J.Physiol.Submitted for publication.

YORITA Katsumi;YAMANO Tetsuo;IKEDA Kazuhiko;KOBAYASH Takashi;SUGANO Tsukasa：Am.J.Physiol.已提交出版。