Role of Kupffer cell in the regulation of hepatic metabolism.

库普弗细胞在肝脏代谢调节中的作用。

• 批准号: 03660309
• 负责人: SUGANO Tsukasa
• 金额: $ 1.54万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660309/
• 关键词: Kupffer cell; Hepatic glucose metabolism; Zymosan; Leukotrien; prostaglandin; hepatic sympathetic nerve; Kupffer細胞; プロスタグラジン

Zymosan pellet induced an increase of glucose production and release of prostanoid (PG) into the perfusate. Zymosan pellet-induced responses were inhibited by cyclooxygenase inhibitor. Possible correlation of the PG release and glucose production were r=0.87 (PGF_2) and r=0.83 (PGD_2), respectively. Zymosan sup or mannan induced a biphasic glucose output and suppression of oxygen consumption in perfused rat livers. These changes were inhibited by lipoxygenase inhibitor or leukotriene (LT) D_4 receptor antagonist, but not cyclooxygenase inhibitor. Sequentially infused zymosan sup after mannan infusion showed the desensitization of second responses, indicating that zymosan sup was rich in mannan. The stimulation of hepatic narve stimulation induced an increase of glucose production and was inhibited by cyclooxygenase inhibitor without affecting norepinephrine overflow. These results suggest that zymosan pellet induces glycogenolysis via PG production and that mannose receptor activation causes peptide-LT synthesis.

酵母聚糖沉淀诱导葡萄糖产量增加以及前列腺素（PG）释放到灌注液中。酵母聚糖颗粒诱导的反应被环氧合酶抑制剂抑制。 PG 释放和葡萄糖产生的可能相关性分别为 r=0.87 (PGF_2) 和 r=0.83 (PGD_2)。酵母聚糖或甘露聚糖在灌注的大鼠肝脏中诱导双相葡萄糖输出并抑制耗氧量。这些变化可被脂氧合酶抑制剂或白三烯 (LT) D_4 受体拮抗剂抑制，但不能被环氧合酶抑制剂抑制。甘露聚糖输注后依次输注酵母聚糖sup显示出第二反应的脱敏，表明酵母聚糖sup富含甘露聚糖。肝神经刺激引起葡萄糖产生增加，并被环氧合酶抑制剂抑制，而不影响去甲肾上腺素溢出。这些结果表明，酵母聚糖颗粒通过 PG 产生诱导糖原分解，并且甘露糖受体激活导致肽-LT 合成。

项目成果

K.Kimura,M.Shiota,K.Mochizuki,M.Ohta and T.Sugano.: "Differnet preparations of zymosan induce glycogenolysis independenctly in the perfused rat liver." Biochem.J.283. 773-779 (1992)

K.Kimura、M.Shiota、K.Mochizuki、M.Ohta 和 T.Sugano.：“不同的酵母聚糖制剂在灌注的大鼠肝脏中独立诱导糖原分解。”

MASAKAZU SHIOTA,YUKI KURANO,YOSHITAKA MOCHIZUKI,KAZUHIRO KIMURA,and TSUKASA SUGANO: "Effects of nerve stimulation and zymosan on glyoogenolysis in perfused livers from coldーexposed rats." Am.J.Physiol.,Gastrointestinal and Liver Physiology.

MASAKAZU SHIOTA、YUKI KURANO、YOSHITAKA MOCHIZUKI、KAZUHIRO KIMURA 和 TSUKASA SUGANO：“神经刺激和酵母聚糖对冷暴露大鼠灌注肝脏糖原分解的影响。”，Am.J.Physiol.，胃肠道和肝脏生理学。

M.Shiota,Y.Kurano,Y.Mochizuki,K.Kimura,M.Ohta and T.Sugano.: "Effects of nerve stimulation and zymosan on glycogenolysis in perfused livers from cold-exposed rats." Am.J.Physiol.263. G353-G359 (1992)

M.Shiota、Y.Kurano、Y.Mochizuki、K.Kimura、M.Ohta 和 T.Sugano.：“神经刺激和酵母聚糖对冷暴露大鼠灌注肝脏糖原分解的影响。”

K.Kimura,H.Shiota,M.Ohta and T.Sugano.: "Different preparations of zymosan induce glycogenolysis independenctly in the perfused rat liver." Biochem.J.283. 773-779 (1992)

K.Kimura、H.Shiota、M.Ohta 和 T.Sugano.：“不同的酵母聚糖制剂在灌注的大鼠肝脏中独立诱导糖原分解。”

H.Shiota,K.Kimura,H.Ohta and T.Sugano.: "Effects of nerve stimulation and zymosan on glycogenolysis in perfused livers from cold-exposed rats." Am.J.Physiol.263. G353-G359 (1992)

H.Shiota、K.Kimura、H.Ohta 和 T.Sugano.：“神经刺激和酵母聚糖对冷暴露大鼠灌注肝脏中糖原分解的影响。”