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Functional Correlations Between Hypothalamus and Hepatic Metabolism

下丘脑与肝脏代谢之间的功能相关性

基本信息

批准号：
01560327
负责人：
SUGANO Tsukasa
金额：
$ 0.96万
依托单位：
College of Agriculture, University of Osaka Prefecture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

The actions of hepatic sympathetic nerves on glycogenolysis were studied in rat livers perfused in situ without recirculation. A bipolar platinum electrode was placed around the hepatic hilus and rectanglar monophasic impulses (20V, 2ms, 20Hz) were applied. In female rat livers both phenylephrine and isoproterenol stimulated glycogenolysis, but the effects of nerve stimulation (NS) were completely abolished by phentolamine. Mepacrine, a phospholipase A_2 inhibitor, markedly suppressed glycogenolysis by NS without affecting norepinephrine overflow. It had no effect, however, on glycogenolysis by phenylephrine. Prostaglandin F_<2alpha> (PGF_<2alpha>) mimicked the effect of NS but phentolamine had no effect on PGF_2. These results suggest that in rat livers eicosanoids produced via an alpha-receptor mediated function play a vital role in the activation of glycogenolysis by NS. Since it has been reported that zymosan (Cell wall particles from yeast) is selectively taken up by kupffer cells and that zymosan is able to stimulate production of eicosanoids, the experiments were carried out to examine the direct effects of sympathetic nerve stimulation and zymosan on glycogenolysis in perfused liver isolated from rats exposed to cold. The results suggest that hepatic nerve may regulate the arachidonic pathways in kupffer cells during cold exposure.

在无再循环的原位灌流大鼠肝脏上，研究了肝交感神经对糖原分解的作用。将双极铂电极置于肝门周围，施加矩形单相脉冲（20 V，2 ms，20 Hz）。在雌性大鼠肝脏中，苯肾上腺素和异丙肾上腺素刺激糖原分解，但神经刺激（NS）的影响完全取消酚妥拉明。磷脂酶A_2抑制剂Macacrine可显著抑制NS引起的糖原分解，但不影响去甲肾上腺素溢出。然而，它对苯丙酮酸的糖原分解没有影响。前列腺素F_<2alpha>（PGF_2<2alpha>）与NS相似，而酚妥拉明对PGF_2无影响。这些结果表明，在大鼠肝脏中，通过α-受体介导的功能产生的类花生酸在NS激活糖原分解中起着至关重要的作用。由于已经报道酵母聚糖（来自酵母的细胞壁颗粒）被枯否细胞选择性摄取并且酵母聚糖能够刺激类二十烷酸的产生，因此进行实验以检查交感神经刺激和酵母聚糖对从暴露于寒冷的大鼠分离的灌注肝脏中糖原分解的直接影响。结果提示，冷暴露时肝神经可能参与了枯否细胞花生四烯酸通路的调节。