Variation of eicosanoid production by activation of Kupffer cells.

库普弗细胞激活导致类二十烷酸产生的变化。

• 批准号: 06660385
• 负责人: SUGANO Tsukasa
• 金额: $ 1.34万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660385/
• 关键词: Kupffer cell; Prostaglandin; Phospholipase A_2; PMA; Thioacetamide; Cirrhosis; Kupffer細胞; プロスタグランジン; PMA; thioacetamide; 肝硬変; エイコサノイド; ロイコトリエン; ホスホリパーゼC; ホルボールミリステートアセテート

Since phorbol ester mimics the actions of LPS,we investigated the metabolic alternation by zymosan-pellet after infusion of phorbol 12-myristate 13-acetate (PMA) in the perfused rat livers. PMA-infusion enhanced the production of Tx B_2 and PGD_2 by zymosan-pellet and arachidonic acid.The response of hepatic macrophages and the effects of platelet-activating factor in perfused liver were studied during production of experimental liver cirrhosis induced by thioacetamide in female Sprague-Dawley rats. Within four weeks of thioacetamide administration (300mg/L drinking water) , an increase in hepatic macrophage population and enlargement in cell size preceded the alterations characteristic of cirrhosis. The amount of PGD_2 and TxB_3 in perfusate induced by platelet-activating factor were seven-and five-fold greater, respectively, in thioacetamide-treated than in control rats. Zymosan mimicked the effects of platelet-activating factor. These results are consisted with the hypothesis that hepatic macrophages and platelet-activating factor play important roles in the development cirrhosis induced by thioacetamide.

由于佛波酯模拟LPS的作用，我们研究了在灌注的大鼠肝脏中注入佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）后酵母多糖颗粒的代谢变化。本实验用硫代乙酰胺诱导雌性SD大鼠肝硬化模型，观察了肝巨噬细胞的反应及血小板活化因子（PAF）的影响。在硫代乙酰胺给药（300 mg/L饮用水）四周内，肝巨噬细胞数量增加和细胞大小增大先于肝硬化特征性改变。硫代乙酰胺组灌流液中血小板活化因子诱导的PGD_2和TxB_3含量分别是对照组的7倍和5倍。酵母聚糖模拟血小板活化因子的作用。这些结果与肝巨噬细胞和血小板活化因子在硫代乙酰胺诱导的肝硬化发生发展过程中起重要作用的假说相一致。

项目成果

K.Kimura,M.Ohta and T.Sugano.: "Phorbol ester altered phagocytosis-induced glycogenolysis by activating thromboxane production in perfused rat livers." Cells of the Hepatic Sinusoid.5.(印刷中). (1995)

K. Kimura、M. Ohta 和 T. Sugano.：“佛波酯通过激活灌注大鼠肝脏中血栓素的产生来改变吞噬作用诱导的糖原分解。”5（出版中）。

K.Kimura,M.Ohta T.Sugano.: "Phorbol ester altered phagocytosis-induced glycogenolysis by activating thromboxane production in perfused rat livers." Cells of the Hepatic Sinusoid. 5. 370-371 (1995)

K.Kimura、M.Ohta T.Sugano.：“佛波酯通过激活灌注大鼠肝脏中血栓素的产生来改变吞噬作用诱导的糖原分解。”

K.Kimura,M.Ohta,T.Sugano.: "Phorbol ester altered phagocytosis-induced glycogenolysis by activating thromboxane production in perfused rat livers." Cells of the IIepatic Sinusoid. 5. 370-371 (1995)

K.Kimura、M.Ohta、T.Sugano.：“佛波酯通过激活灌注大鼠肝脏中血栓素的产生来改变吞噬作用诱导的糖原分解。”

S.Noda,S.Masumi,M.Moriyama,M.Ohta,Y.Kannan,T.Sugano.: "Population of hepatic macrophages and response of perfused liver to platelet-activating factor during production of thioacetoamide-induced cirrhosis in rats." Hepatology,. (in press). (1996)

S.Noda、S.Masumi、M.Moriyama、M.Ohta、Y.Kannan、T.Sugano.：“在大鼠硫代乙酰胺诱导的肝硬化过程中，肝巨噬细胞的数量以及灌注肝脏对血小板激活因子的反应。

S.Noda,S.Masumi,M.Moriyama,M.Ohta,Y.Kannan,T.Sugano.: "Population of hepatic macrophages and response of perfused liver to platelet activating factor during production of thioacetoamide-induced cirrhosis in rats." Hepatology,. (in press).

S.Noda，S.Masumi，M.Moriyama，M.Ohta，Y.Kannan，T.Sugano.：“在大鼠硫代乙酰胺诱导的肝硬化过程中，肝巨噬细胞的数量以及灌注肝脏对血小板激活因子的反应。”