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Glutamate receptor-channels induced by brain messenger RNA in Xenopus oocytes

非洲爪蟾卵母细胞中脑信使 RNA 诱导的谷氨酸受体通道

基本信息

批准号：
60570058
负责人：
KURODA Hideyo
金额：
$ 1.02万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570058/
关键词：
mRNA Xenopus oocyte glutamate receptor receptor-channel カイニン酸

项目摘要

The glutamate receptor-channels induced in the membrane of Xenopus laevis oocyte injected with mRNA extracted from cerebral cortex, striatum and hippocampus in the rat brain and the bovine brain were investigated electrophysiologically.(1) The oocyte resting membrane potential ranged from -50 to -60 mV. The changes in the membrane potentials were induced in 10^<-6>M concentration of glutamate and its analogues, N-methyl-D-aspartate, quisqualate and kainate. The depolarizing responses were mostly observed, but some-times the hyperpolarizing responses occurred.(2) The differences in the induction of receptor-channels were not found between mRNAs extracted from cerebral cortex, striatum and hippocampus, and between the rat brain and the bovine brain.(3) Kainate always depolarized the membranes, in a dose-dependent manner, with threshold concentration as low as 10^<-8>M. The oocyte responses to kainate were separated into the three distinct types: Type 1, the fast depolarization without repolarization for a long period; Type 2, the spike-like depolarization followed by slow depolarization in the shape of plateau; Type 3, the spike-like depolarization.(4) The antagonists to the excitatory amino acid, D- -adipate,2-amino-4-phosphonobutyric acid, glutamate diethylester and -D-glutamylglycine did not inhibit the three types of responses to kainate.(5) The responses to kainate were not sensitized.

用电生理学方法研究了大鼠和牛大脑皮层、纹状体和海马的mRNA对非洲爪蟾卵母细胞膜上谷氨酸受体通道的诱导作用。(1)卵母细胞静息膜电位范围为-50至-60 mV。在10 μ <-6>M浓度的谷氨酸及其类似物，N-甲基-D-天冬氨酸，使君子酸和红藻氨酸诱导膜电位的变化。主要观察到去极化反应，但有时也出现超极化反应。(2)从大脑皮层、纹状体和海马提取的mRNA之间以及大鼠脑和牛脑之间未发现受体通道诱导的差异。(3)红藻氨酸盐总是以剂量依赖性方式使膜去极化，阈值浓度低至10 μ <-8>M。卵母细胞对红藻氨酸的反应可分为三种不同的类型：1型，快速去极化，长时间不复极化; 2型，尖峰状去极化，随后出现平台状缓慢去极化; 3型，尖峰状去极化。(4)兴奋性氨基酸的拮抗剂，D-己二酸，2-氨基-4-膦酰基丁酸，谷氨酸二乙酯和-D-谷氨酰甘氨酸不抑制红藻氨酸的三种类型的反应。(5)对红藻氨酸的反应不敏感。