Sperm factor caused a transient increase in intracellular calcium concentration of sea urchin eggs.

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RESEACH PLANWe have shown that the application of soluble sperm extract elicits a Ca^<2+>-transient in fertilized, denuded eggs of sea urchin. In this project, we will try to purify this sperm factor and to elucidate how the factor causes the transient.RESULTSA sperm factor was partially purified using column chromatography of cation exchanger-anion exchanger-amido 80. The activity was stable in acid, alkali and protease. But we could not succeed further purification. The activity did not probably depend on a single factor.The Ca^<2+>-transient by sperm extract was inhibited by heparine or GDP-betaS unlike one during fertilization. Furthermore, sperm extract caused a transient increase in IP_3 mass but not in cGMP mass. These results show that sperm extract caused IICR in fertilized eggs but not stimulate CICR.