Sperm factor caused a transient increase in intracellular calcium concentration of sea urchin eggs.

精子因子导致海胆卵细胞内钙浓度短暂升高。

• 批准号: 05640764
• 负责人: KURODA Hideyo
• 金额: $ 1.34万
• 依托单位: Toyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640764/
• 关键词: sea urchin; egg; calcium ion; sperm; inositol 1,4,5-trisphosphate; cGMP; Inositol 1,4,5-Trisphosphate; cGMP

RESEACH PLANWe have shown that the application of soluble sperm extract elicits a Ca^<2+>-transient in fertilized, denuded eggs of sea urchin. In this project, we will try to purify this sperm factor and to elucidate how the factor causes the transient.RESULTSA sperm factor was partially purified using column chromatography of cation exchanger-anion exchanger-amido 80. The activity was stable in acid, alkali and protease. But we could not succeed further purification. The activity did not probably depend on a single factor.The Ca^<2+>-transient by sperm extract was inhibited by heparine or GDP-betaS unlike one during fertilization. Furthermore, sperm extract caused a transient increase in IP_3 mass but not in cGMP mass. These results show that sperm extract caused IICR in fertilized eggs but not stimulate CICR.

研究计划我们已经证明，应用可溶性精子提取物可引起海胆受精卵中Ca^<2+>-瞬变。在本项目中，我们将尝试纯化该精子因子，并阐明该因子是如何引起瞬变的。结果采用阳离子交换器-阴离子交换器-氨基80柱层析法对sa精子因子进行了部分纯化。在酸、碱和蛋白酶中活性稳定。但我们无法进一步提纯。这种活动可能不取决于单一因素。精子提取物的Ca^<2+>-瞬变不像受精时那样被肝素或gdp - β抑制。此外，精子提取物引起IP_3质量的短暂增加，而cGMP质量没有增加。这些结果表明，精子提取物引起受精卵的IICR，但不刺激CICR。

项目成果

M.Osawa: "Soluble sperm extract triggers inositol 1,4,5-Trisphosphate-induced Ca^<2+> release in the oocytes of the sea urchin,Anthocidaris crassispina" Cell Struct.Funct.19. 73-80 (1994)

M.Osawa：“可溶性精子提取物触发海胆 Anthocidaris crassispina 卵母细胞中肌醇 1,4,5-三磷酸诱导的 Ca^2 释放”Cell Struct.Funct.19。

M.Osawa: "Sperm and its soluble extract cause transient incr eases in intracellular calcium concertration and inmembr ane pot ential of sea ur chinzygotes." Dev.Biol.166. 268-276 (1994)

M.Osawa：“精子及其可溶性提取物会导致海胆合子的细胞内钙浓度和膜内电位短暂增加。”

M.Osawa: "Sperm and its soluble extract cause Transient increases in intacellular calcium concentration and in membrane potential of sea urchin zygotes" Dev.Biol.166. 268-276 (1994)

M.Osawa：“精子及其可溶性提取物导致海胆受精卵的细胞内钙浓度和膜电位瞬时增加”Dev.Biol.166。

M.Osawa, N.Kaneko, A.Terakawa, T.Kitani, R.Kuroda and H.Kuroda: "Studies on the mechanism for Ca_i-transient in sea urchin zygotes caused by refertilization and external application of sperm extract" Exptl.Cell Res.(submitted).

M.Osawa、N.Kaneko、A.Terakawa、T.Kitani、R.Kuroda 和 H.Kuroda：“再受精和精子提取物外用引起海胆受精卵 Ca_i 瞬态机制的研究” Exptl.Cell

M.Osawa: "Solude sperm extrace trrigers inositol-1,4,5-trisphosphate induced Ca^<2+> release in the oocyter of the seaurchin,Anthocidar is cr assispina" Cell Struct.Funct.19. 73-80 (1994)

M.Osawa：“溶质精子提取物触发肌醇-1,4,5-三磷酸盐诱导海胆卵母细胞中的 Ca^2 释放，Anthocidar is cr assisspina”Cell Struct.Funct.19。