Sperm factor caused a transient increase in intracellular calcium concentration of sea urchin eggs.

研究计划我们已经证明，应用可溶性精子提取物可引起海胆受精卵中Ca^<2+>-瞬变。在本项目中，我们将尝试纯化该精子因子，并阐明该因子是如何引起瞬变的。结果采用阳离子交换器-阴离子交换器-氨基80柱层析法对sa精子因子进行了部分纯化。在酸、碱和蛋白酶中活性稳定。但我们无法进一步提纯。这种活动可能不取决于单一因素。精子提取物的Ca^<2+>-瞬变不像受精时那样被肝素或gdp - β抑制。此外，精子提取物引起IP_3质量的短暂增加，而cGMP质量没有增加。这些结果表明，精子提取物引起受精卵的IICR，但不刺激CICR。

RESEACH PLANWe have shown that the application of soluble sperm extract elicits a Ca^<2+>-transient in fertilized, denuded eggs of sea urchin. In this project, we will try to purify this sperm factor and to elucidate how the factor causes the transient.RESULTSA sperm factor was partially purified using column chromatography of cation exchanger-anion exchanger-amido 80. The activity was stable in acid, alkali and protease. But we could not succeed further purification. The activity did not probably depend on a single factor.The Ca^<2+>-transient by sperm extract was inhibited by heparine or GDP-betaS unlike one during fertilization. Furthermore, sperm extract caused a transient increase in IP_3 mass but not in cGMP mass. These results show that sperm extract caused IICR in fertilized eggs but not stimulate CICR.