Role of renal afferent nerve in the regulation of blood pressure

肾传入神经在血压调节中的作用

• 批准号: 60570091
• 负责人: ABE Youichi
• 金额: $ 0.96万
• 依托单位: Kagawa Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570091/
• 关键词: Afferent renal nerve; Regulation of blood pressure; Nerve stimulation; Hypertension; ラット

The kidney plays an important role in the regulation of systemic blood pressure via the metabolism of water and sodium, and via humoral factors such as the renin-angiotensin system. However, there is little information about the role of the renal afferent nerve. The present study was designed to clarify the role of the renal afferent nerve in the regulation of blood pressure, using Wistar-Kyoto rats (WKY) and spontaneously hypertensive rat (SHR).[Methods] Thirteen to 15-week-old WKY and SHR were used in this investigation. Under pentobarbital anesthesia, the left renal sympathetic nerves were exposed through a midline abdominal incision. Rectangular pulses were delivered to the renal nerve. The frequency was varied between 1 and 20 Hz, while the voltage and duration of stimulation were held constant (10 V and 1 msec, respectively). For recording of renal afferent nerve activity, the renal nerve was sectioned, the peripheral end of the sectioned nerve placed on electrodes, and the actio … More n potential recorded.[Results and Discussion] 1. Effects of renal afferent nerve stimulation on blood pressure: Renal afferent nerve stimulation at variable frequencies for 20 sec produced a fall in blood pressure in both WKY and SHR in a frequency-dependent fashion. At high frequencies (5-20 Hz) the depressor responses were not different between WKY and SHR, but at low frequencies (1-2 Hz) the responses were significantly smaller in SHR than in WKY. These depressor responses were completely abolished after spinal cord section at C4 or T8, but not after section at L2. It can be concluded that the reflex may be centrally reset in SHR. Thus, the renal afferent cell bodies may be located in the T9-L2 ganglion.2. Recording of renal afferent nerve activity: Since resting afferent nerve discharge is very low, it was difficult to distinguish from noise. However, nerve discharge was significantly increased during partial occlusion of the renal vein. An increase in ureteral pressure did not affect nerve discharge. These results indicate that the renal mechanoreceptor was not stimulated by these changes in renal tissue pressure, so the mechanoreceptor might be located in the intrarenal venous system. Elevation of renal venous pressure increased discharge to similar extent in both WKY and SHR. Less

肾脏通过水和钠的代谢，以及肾素-血管紧张素系统等体液因子，在调节全身血压方面发挥着重要作用。然而，关于肾传入神经的作用的信息很少。本研究以Wistar-Kyoto大鼠（WKY）和自发性高血压大鼠（SHR）为研究对象，探讨肾传入神经在血压调节中的作用。[方法]采用13 ~ 15周龄的WKY和SHR进行调查。戊巴比妥麻醉下，左肾交感神经经腹部中线切口显露。矩形脉冲被传送到肾神经。频率在1到20 Hz之间变化，而刺激的电压和持续时间保持不变（分别为10 V和1毫秒）。为了记录肾传入神经的活动，将肾神经切开，将切开的外周端置于电极上，记录肾传入神经的活动。【结果与讨论】肾传入神经刺激对血压的影响：在不同频率下刺激肾传入神经20秒，WKY和SHR的血压都以频率依赖的方式下降。在高频率（5 ~ 20 Hz）下，WKY和SHR的抑制反应无显著差异，但在低频率（1 ~ 2 Hz）下，SHR的抑制反应明显小于WKY。这些抑制反应在C4或T8脊髓切片后完全消失，但在L2脊髓切片后则没有。可以得出结论，反射可能在SHR中被集中重置。因此，肾传入细胞体可能位于T9-L2神经节。肾传入神经活动记录：由于静息传入神经放电极低，难以与噪音区分。然而，在肾静脉部分闭塞时，神经放电明显增加。输尿管压力升高不影响神经放电。这些结果表明，肾组织压力的变化没有刺激肾机械感受器，因此机械感受器可能位于肾内静脉系统。肾静脉压升高使WKY和SHR患者的排出量增加的程度相似。少

项目成果

Y. Abe: "Role of a renal afferent nerve in the regulation blood pressure in rats: Modulation by the vasal levels of systemic blood pressure."

Y. Abe：“肾传入神经在大鼠血压调节中的作用：全身血压的血管水平的调节。”

S. Fujioka: "Effects of a synthetic human atrial natriuretic polypeptide on regional blood flow in rats." Europ. J. Pharmacol.109. 301-304 (1985)

S. Fujioka：“合成的人心房钠尿多肽对大鼠局部血流的影响。”

T. Tamaki: "Effects of ouabain on autoregulation of renal blood flow in dogs." Am. J. Physiol.250. F109-F114 (1986)

T. Tamaki：“哇巴因对狗肾血流自动调节的影响。”

S.Fujioka: Europ.J.Pharmacol.109. 301-304 (1985)

S.Fujioka：Europ.J.Pharmacol.109。

S.Koyama: Europ.J.Pharmacol.127. 43-48 (1986)

S.Koyama：Europ.J.Pharmacol.127。