Studies on the Modifications of Some Screening Methods for Health Examination in Lead Workers

主管人员健康检查部分筛查方法的修改研究

• 批准号: 60570243
• 负责人: TOMOKUNI Katsumaro
• 金额: $ 1.09万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570243/
• 关键词: Lead-Exposed Worker; Specific Examination; Modification; Urinary <delta> -Aminolevulinic Acid; Urinary Coproporphyrin; 血中鉛

The determinations of urinary <delta> -aminolevulinic acid (ALA), urinary coproporphyrin (COPRO) and blood lead are useful in evaluating the health effect by lead in workers occupationally exposed to lead. The present study was undertaken to simplify some screening methods being utilized for checking the health effect in lead workers. The results were as follows:1. Determination of urinary ALA; We modified an original method of Tomokuni and Ogata. When urinary ALA was determined using the original method of Tomokuni and Ogata after 3-fold dilution of urine with distilled water, it was possible to eliminate the urinary matrix interference. As for the condensation reagent for ALA-pyrrole, methyl acetoacetate was superior to ethyl acetoacetate.2. Determination of urinary COPRO; The conventional methods (Rimington method and its modification) based on sample extraction and the spectrophotometric measurement at the peak of the Soret band for COPRO are very time-consuming. In the present study, we developed a simplified method for determining urinary COPRO in lead workers, using a high-performance liquid chromatograph (HPLC) equipped with a fluorescence detector. The detection limit of urinary COPRO in this method was 5 ug per liter of urine. The working linear range of urinary COPRO concentration was 5 to 1500 ug/L. This HPLC method is simpler and more rapid than the spectrophotometric method and is useful in mass screening for lead exposure.3. Determination of blood lead; We modified a method of Hodges and Skelding for determining blood lead by flameless atomic absorption spectrophotometry. In the present method, a graphite tube was coated with molybdenum and both Triton X-100 and phosphoric acid were added to whole blood. The calibration curve was made by adding standard lead solution to the pooled blood of normal controls. This method was more reliable compared to the original method.

尿β-氨基乙酰丙酸(ALA)、尿钴卟啉(COPRO)和血铅的测定对职业性铅接触工人的健康影响有一定的参考价值。本研究旨在简化铅作业工人健康影响的筛查方法。结果如下：1.尿ALA测定：我们对Tomokuni和Ogata的原方法进行了改进。尿液用蒸馏水稀释3倍后，用Tomokuni和Ogata原方法测定尿ALA，可消除尿基质干扰。在丙氨酸-吡咯的缩合试剂中，乙酰乙酸甲酯优于乙酰乙酸乙酯。尿铜的测定，传统的方法(里明顿法及其改进法)是建立在样品提取和索雷特谱峰分光光度测定的基础上，非常耗时。在本研究中，我们建立了一种简易的测定铅作业工人尿铜的方法，使用配有荧光检测器的高效液相色谱仪(HPLC)。该方法对尿铜的检出限为每升尿5微克。尿铜浓度在5~1500 ug/L范围内呈良好的线性关系。该方法比分光光度法简便、快速，可用于铅暴露的大范围筛查。血铅的测定：对Hodges和Skding的无焰原子吸收光谱法测定血铅的方法进行了改进。在本方法中，在石墨管上包覆钼，并在全血中同时加入Triton X-100和磷酸。将标准铅溶液加入正常对照的混合血中，制成校正曲线。与原方法相比，该方法更加可靠。

项目成果

Tomokuni Katsumaro: "Factors Affecting Determination of <delta> -Aminolevulinate by Use of Ehrlich's Reagent" Clinical Chemistry. 32. 192-193 (1986)

Tomokuni Katsumaro：“影响使用艾利希试剂测定<δ>-氨基乙酰丙酸盐的因素”临床化学。

Tomokuni Katsumaro: "Effect of Urea on the Simple Determination of <delta> -Aminolevulinic Acid in Urine" Industrial Health. 23. 295-297 (1985)

Tomokuni Katsumaro：“尿素对尿液中<δ>-氨基乙酰丙酸简单测定的影响”工业健康。

Koga Naomi: "Direct Determination of Lead in Blood by Flameless Atomic Absorption Spectrophotometry" Japanese Journal of Hygiene. 40. 636-640 (1985)

Koga Naomi：“通过无焰原子吸收分光光度法直接测定血液中的铅”日本卫生杂志。

Tomokuni Katsumaro: Clinical Chemistry. 32. 192-193 (1986)

Tomokuni Katsumaro：临床化学。

Tomokuni Katsumaro: Clinical Chemistry. 32. 872-873 (1986)

Tomokuni Katsumaro：临床化学。