New indicators useful for biological monitoring of organic solvent and lead exposures

可用于有机溶剂和铅暴露生物监测的新指标

• 批准号: 07557218
• 负责人: TOMOKUNI Katsumaro
• 金额: $ 0.83万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557218/
• 关键词: biological monitoring; urinary metabolite; trimethylbenzene exposure; dimethylhippuric acid; lead exposure; plasma delta-aminolevulinic acid; 暴露評価; トリメチルベンゼン

Trimethylbenzene (TMB) is used in industry as solvents and paint thinners.The threshold limit value (TLV) of TMB has been set at 25 ppm (ACGIH) and, however, its biological exposure index (BEI) has not been recommended yet. TMB consists of three isomers ; 1,2,3-, 1,2,4-, and 1,3,5-trimethylbenzene. Of these isomers, 1,2,4-TMB (pseu-documene) is mainly contained in the TMB solvent. It is known that 1,2,4-TMB is mainly metabolized to 3,4-dimethylhippuric acid (3,4-DMHA) and its metabolite is excreted into urine in rats.In the present study, we first examined the urinary excretion of 3,4-DMHA in workers occupationally exposed to 1,2,4-TMB and evaluated whether it is useful as a biological indicator of 1,2,4-TMB exposure. The data obtained indicated that the concentration of urinary 3,4-DMHA had a positive correlation with the level of exposure to 1,2,4-TMB in workers. From this result, we confirm that urinary 3,4-DMHA is one of the most useful indicators for biological monitoring of 1,2,4-TMB exposure.Secondly, we investigated the relationship between plasma delta-aminolevulinic acid (ALA) concentration and the degree of exposure to lead in about 400 lead workers.The data obtained indicated that plasma ALA concentration was one of the most useful indicators for the biological monitoring of occupational exposure to lead.

三甲苯 (TMB) 在工业上用作溶剂和油漆稀释剂。TMB 的阈限值 (TLV) 已设定为 25 ppm (ACGIH)，但其生物暴露指数 (BEI) 尚未推荐。 TMB由三种异构体组成； 1,2,3-、1,2,4-和1,3,5-三甲苯。在这些异构体中，TMB溶剂中主要含有1,2,4-TMB(拟多枯烯)。众所周知，1,2,4-TMB 在大鼠体内主要代谢为 3,4-二甲基马尿酸（3,4-DMHA），其代谢物排泄到尿液中。在本研究中，我们首先检测了职业接触 1,2,4-TMB 的工人尿液中 3,4-DMHA 的排泄情况，并评估其是否可作为 1,2,4-TMB 的生物学指标。 1,2,4-TMB 暴露。所得数据表明，工人尿液中3,4-DMHA浓度与1,2,4-TMB暴露水平呈正相关。由此结果，我们确认尿中3,4-DMHA是1,2,4-TMB暴露生物监测最有用的指标之一。 其次，我们调查了约400名铅工人血浆δ-氨基乙酰丙酸（ALA）浓度与铅暴露程度之间的关系。 获得的数据表明，血浆ALA浓度是1,2,4-TMB暴露生物监测最有用的指标之一。 职业接触铅。

项目成果

Hirotaka Oishi: "Fluorometric HPLC determination of delta-aminolevulinic acid (ALA) in the plasma and urine of lead workers : Biological indicators of lead exposure" J Anal Toxicol. 20. 106-110 (1996)

Hirotaka Oishi：“荧光 HPLC 测定铅工人血浆和尿液中的 δ-氨基乙酰丙酸 (ALA)：铅暴露的生物指标”J Anal Toxicol。

友国勝麿: "尿中δ-アミノレブリン酸(スクリーニング法)" 日本臨床. 53. 1383-1388 (1995)

Katsumaro Tomokuni：“尿δ-氨基乙酰丙酸（筛选方法）”日本临床研究 53. 1383-1388（1995）。

Katsumaro Tomokuni: "delta-Aminolevulinic acid (screening method)" NIHON RINSYO. 53. 1383-1388 (1995)

Katsumaro Tomokuni：“δ-氨基乙酰丙酸（筛选方法）”NIHON RINSYO。

H.Oishi: "Fluorometric HPLC determination of δ-aminolevulinic acid (ALA) in the plasma and urine of lead workers ;" J.Anal.Toxicol.20. 106-110 (1996)

H.Oishi：“荧光 HPLC 测定铅工人血浆和尿液中的 δ-氨基乙酰丙酸 (ALA)；J.Anal.Toxicol.20 (1996)。

H.Oishi: "Fluorometric HPLC determination of δ-aminolevulinic acid(ALA) in the plasma and urine of lead workers;" J.Anal.Toxicol.20. 106-110 (1996)

H.Oishi：“荧光 HPLC 测定铅工人血浆和尿液中的 δ-氨基乙酰丙酸 (ALA)；J.Anal.Toxicol.20 (1996)。