Study of improved method for detecting mutagenic activities of volatile and gaseous compounds.

由于气体或挥发性化合物的致突变性尚未确定，我们发展了一种简便的鼓泡法，以氯乙烯和环氧乙烷为例，用100%氯乙烯（VC）在试管中鼓泡10-15分钟，吸收到二甲基亚砜（DMSO）中，用此方法检测这些化合物的致突变性。然后将0.1ml上述DMSO、0.1ml新鲜培养的试验菌株（大肠杆菌WP 2uvrA/pKM）和0.5ml S9 Mix在气密小瓶中混合，在37 ℃下振荡30-120分钟，并与2 ml顶层琼脂一起倒在最小葡萄糖琼脂平板上。在37 ℃孵育48小时后对Trp回复突变体进行评分.另一方面，用气相色谱法测定上述DMSO中VC的浓度，并用此方法进行环氧乙烷（EO）（12%）的致突变性试验。EO被吸收到10 ml乙醇中。使用不含S9 Mix和预孵育的鼠伤寒沙门氏菌TA 100，通过相同方法进行EO致突变性试验。用气相色谱法对环氧乙烷进行了定量分析，结果表明VC和EO均呈阳性，且有剂量-反应关系。VC和EO的回归曲线分别为Y=0.2X + 205.3和Y=0.157X + 157.3（p <0.001）。

As the mutagenicity for gaseous or volatile compounds has not been established, we have developed a simple method by bubbling for detecting the mutagenic activities in these compounds.Vinyl chloride and ethylene oxide, for example, were tested by this simple method.Vinyl chloride(VC)(100%) was absorbed into dimethylsulfoxide(DMSO) in a tube by bubbling for 10-15 minutes. Then 0.1ml of the above DMSO, 0.1ml of a fresh cultured tester strain (E.coli WP2uvrA/pKM) and 0.5ml of S9Mix were mixed in a gas tight vial, shaked at 37 C for 30-120 minutes and poured with 2ml of top agar onto a minimal glucose agar plate. Trp revertants were scored after incubation at 37 C for 48 hours. On the other hand, the concentration of VC in the above DMSO was measured by gaschromatography.The mutagenicity test of ethylene oxide(EO)(12%) was performed by this method. EO was absorbed into 10ml of ethylalcohol. The mutagenicity test for EO was carried out by the same method using S.typhimurium TA100 without S9Mix and preincubation. The quantitative analysis of EO was performed by gaschromatography.The positive results with dose-response effects were observed both VC and EO. The regression curves are Y=0.2X + 205.3 in VC and Y=0.157X + 157.3 in EO (p 0.001).This bubbling method is useful and simple for detecting mutagenic activities in gaseous compounds, quantitatively.