Biological effects and influence of bio-high molecules of electromagnetic fields

流行病学研究表明，白血病的发病人数增加，不仅是生活在高压电线下的居民，而且是职业性接触电磁场的工人。本研究的目的是探讨静磁场的生物效应。1）研究了5 T静磁场暴露48小时和72小时对动物摄食量和饮水量的影响。观察到48小时暴露组的饮水量和摄食量显著减少。2）对氨基苯酚（p-AP）对大肠杆菌WP 2uvrA/pKM 101有致突变作用。加入DMSO或过氧化氢酶可抑制p-AP的致突变活性。在Fe（III）存在下，p-AP诱导的DNA切割参与了氧化性DNA损伤。我们计划研究电磁场暴露对这一现象的影响。3）据报道，脑肿瘤是由电磁场诱发的流行病学。为了研究这一证据，我们建立了一个新的体外微核试验系统，使用原代培养的星形胶质细胞来源于新生雄性大鼠脑。首先，以丝裂霉素C、长春新碱、环磷酰胺等抗癌药物为研究对象，建立了新的检测体系。得到了充分的结果。电磁场暴露组和非暴露组的诱变效应有待于进一步研究。4）用电磁场和低频磁场对黑腹拟步行虫进行现场诱变试验。

Epidemiological study showed that increased number of leukemia was observed not only residents who were living under the high voltage wire code but also workers who were exposed to electromagnetic fields occupationally. The aim of this studies is to investigate a biological effects of static magnetic fields. The following results were obtained for 2 years investigation.1)The effects of 5 tesla(T) static magnetic fields(SMF) on food and water consumption were examined for 48hr and 72hr exposures. Significant decrease of water and food consumption were observed for 48hr exposure groups. On the other hand, increased tendency of food, water and body weight was observed for 72hr groups.2)p-Aminophenol(p-AP) was mutagenic to E.coli WP2uvrA/pKM101. The mutagenic activity of p-AP was suppressed with the addition of DMSO or catalase. p-AP induced DNA cleavage in the presence of Fe(III)participates in the oxidative DNA damage. We planned to investigate the effects of EMF exposure to this phenomena.3)It is reported that brain tumor is induced by EMF epidemiologically. To investigate this evidence, we established a new in vitro micronucleus test system using primary cultured astrocyte derived from rat newborn male brain. First of all, some anticancer drugs, such as mitomycin C, vincristine and cyclophosphamide, were used to establish a new test system. Sufficient results are obtained. Exposure and non exposure groups to EMF should be studied in next step.4)Mutagenic effects of spot-test using Dorosophila melanogaster were observed by SMF and Low frequency magnetic fields.