Biological effects and influence of bio-high molecules of electromagnetic fields

流行病学研究表明，在高压电线编码下生活的居民和接触电磁场的工作人员患白血病的人数均有增加。本研究的目的是探讨静磁场的生物效应。经过2年的调查，得出以下结果：1)研究了5特斯拉(T)静磁场（SMF）在48小时和72小时下对食物和水消耗的影响。48小时暴露组的水和食物消耗量显著减少。另一方面，72hr组的食物、水和体重有增加的趋势。2)对氨基酚（p-AP）对大肠杆菌WP2uvrA/pKM101具有诱变作用。添加DMSO或过氧化氢酶可抑制p-AP的诱变活性。在Fe（III）存在下p-AP诱导的DNA裂解参与了DNA的氧化损伤。我们计划调查EMF暴露对这种现象的影响。3)流行病学报道电磁场诱发脑肿瘤。为了研究这一证据，我们用原代培养的大鼠雄性新生脑星形胶质细胞建立了一种新的体外微核试验系统。首先，采用丝裂霉素C、长春新碱、环磷酰胺等抗癌药物，建立了新的检测体系。得到了充分的结果。下一步应研究电磁场暴露组和非暴露组。4)利用SMF和低频磁场观察黑腹Dorosophila melanogaster斑点试验的致突变效应。

Epidemiological study showed that increased number of leukemia was observed not only residents who were living under the high voltage wire code but also workers who were exposed to electromagnetic fields occupationally. The aim of this studies is to investigate a biological effects of static magnetic fields. The following results were obtained for 2 years investigation.1)The effects of 5 tesla(T) static magnetic fields(SMF) on food and water consumption were examined for 48hr and 72hr exposures. Significant decrease of water and food consumption were observed for 48hr exposure groups. On the other hand, increased tendency of food, water and body weight was observed for 72hr groups.2)p-Aminophenol(p-AP) was mutagenic to E.coli WP2uvrA/pKM101. The mutagenic activity of p-AP was suppressed with the addition of DMSO or catalase. p-AP induced DNA cleavage in the presence of Fe(III)participates in the oxidative DNA damage. We planned to investigate the effects of EMF exposure to this phenomena.3)It is reported that brain tumor is induced by EMF epidemiologically. To investigate this evidence, we established a new in vitro micronucleus test system using primary cultured astrocyte derived from rat newborn male brain. First of all, some anticancer drugs, such as mitomycin C, vincristine and cyclophosphamide, were used to establish a new test system. Sufficient results are obtained. Exposure and non exposure groups to EMF should be studied in next step.4)Mutagenic effects of spot-test using Dorosophila melanogaster were observed by SMF and Low frequency magnetic fields.