Effects of exposures to various air pollutants on experimental asthma induction, in particular on its late asthmatic response.

暴露于各种空气污染物对实验性哮喘诱发的影响，特别是对其晚期哮喘反应的影响。

• 批准号: 60570253
• 负责人: YOSHIDA Katsumi
• 金额: $ 1.02万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570253/
• 关键词: Nitrogen Dioxide; Airway Sensitization; Breathing Curve; Late Asthmatic Response; Air Pollution; 気管支喘息

On bronchial asthma, it is pointed out that late asthmatic response (LAR) is important and its significance is large, but its adequate identification/experimental procedure has not been developped for the difficulty of objective long-time observation (over 48 hours) on experimental animals' breathing symptoms.In the first step of our project, new procedure of long-time exact observation on breathing symptoms was planned to be developped. Animals (guinea pigs) were fixed in bodyplethsmo-boxes, and each output was amplified, converted to digital data, and stored in hard-disk. Stored data were analized numerically to obtain the following indices of respiratory frequency, ecpiration/ inspiration-ratio (expiratory/inspiratory elongation), breathing curve pattern, power spectrum of breathing curve etc.With this system, effect of nitrogen dioxide on LAR was investigated. By ovo-albumin or candidaextract spray, it was noticed that LAR was elevated siginificantly by exposure to nitrogen dioxide (0.5 ppm X 24 hours X 4 days).

在支气管哮喘方面，指出晚期哮喘反应（late asthma response， LAR）很重要且意义重大，但由于实验动物的呼吸症状难以长时间客观观察（超过48小时），尚未形成适当的识别/实验程序。在项目的第一步，我们计划开发长时间精确观察呼吸症状的新程序。动物（豚鼠）被固定在身体体积盒中，每一次输出都被放大，转换成数字数据，并存储在硬盘中。对存储的数据进行数值分析，得到呼吸频率、吸气/吸气比（呼气/吸气伸长率）、呼吸曲线模式、呼吸曲线功率谱等指标，并利用该系统研究二氧化氮对LAR的影响。通过卵白蛋白或念珠菌提取物喷雾，发现暴露于二氧化氮（0.5 ppm X 24小时X 4天）可显著升高LAR。

项目成果

吉田克巳: アレルギー. (1987)

吉田克己：过敏 (1987)

K. Yoshida, M. Kitabatake, N. Uchida et al: "Numerical Analysis with Personal Computer on Breathing Curves obtained from experimentally asthma-induced Guinea Pigs" Report of Environmental Science of Mie University. 10. 119-124 (1985)

K. Yoshida、M. Kitabatake、N. Uchida 等人：“用个人计算机对实验诱发哮喘的豚鼠获得的呼吸曲线进行数值分析”三重大学环境科学报告。

吉田克巳: 三重大学環境科学研究紀要. 10. 119-124 (1985)

吉田克己：三重大学环境科学研究通报。10. 119-124 (1985)

K. Yoshida, M. Kitabatake and N. Uchida: "Identification and Recording of experimental Asthma LAR with personal Computer" Allergy.

K. Yoshida、M. Kitabatake 和 N. Uchida：“用个人计算机识别和记录实验性哮喘 LAR”过敏。