Study on regulatory mechanism of liver regeneration

暴发性肝炎是一种众所周知的高致病性疾病，本研究旨在开发治疗该病的新模式。也就是说，在这种情况下，为了提高存活率，毫无疑问，促进肝脏再生对于携带大量肝坏死的肝脏是绝对必要的。因此，对肝再生的调控机制及其在重型肝炎治疗中的应用进行了研究。在未来的几年里，我们可以开发新的方法来分析肝脏再生的单克隆抗溴脱氧尿苷抗体。在过去的研究中，我们明确了胰高血糖素和胰岛素同时输注不仅对部分去肝的大鼠肝脏，而且对严重受损的大鼠肝脏也有促进肝脏再生的作用。结果表明，由大肠杆菌产生的人表皮生长因子与其他两种胰腺激素合用对肝脏再生的促进作用更大。这一结果可能是开发暴发性肝炎治疗新模式的关键。此外，测定包括暴发性肝炎在内的杂性肝病患者血清中人表皮生长因子的含量也很重要。我们还成功地利用抗hEGF酶免疫分析法建立了该检测系统。

This study has been aimed to develop new modality in therapy of fulminant hepatitis which is well-known to be disease showing very high modality. That is, in order to improve the survival rate in this condition it is no doubt that promotion of liver regeneration will be absolutely necessary in the liver carrying massive liver necrosis. Therefore, studies concerning regulatory mechanism of liver regeneration and its application to the therapy of fulminant hepatitis has been performed. During due years, we could developed new method for analysis of liver regeneration using monoclonal anti-bromodeoxy uridine antibody. In the past study, we made clear that simultaneous infusion of glucagon and insulin promoted liver regeneration not only in partial hepatectomized rat liver, but also in the severely damaged liver. However, we could add the new evidence that human epidermal growth factor which was produced by E. Coli promoted the liver regeneration much more in its concomitant use with other two pancreatic hormones. This result might be key for development of new therapeutic modality of fulminant hepatitis. Furthermore, it is also important to measure the content of human epidermal growth factor in the sera among the cases carrying miscellaneous liver diseases including fulminant hepatits. We also succeeded to this assay system using enzyme immunoassay usin anti hEGF.