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In vivo study of radioimmunodetection and radioimmunotherapy with I-131 labeled monoclonal antimelanoma antibody

I-131标记单克隆抗黑色素瘤抗体放射免疫检测和放射免疫治疗的体内研究

基本信息

批准号：
60570486
负责人：
KUSAKABE Kiyoko
金额：
$ 0.9万
依托单位：
Tokyo Women's Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

(1) Radioisotope labeled monoclonal andibodies are going to use for imaging and treatment of disseminated malignant tumors.(2) This study was undertaken in the differrent distributions of anti-melanoma antibodis(AMAB) in the mouse melanoma.(3) The moenoclonal antibodies were obtained by fusion of BALB/C myeloma and C57BL/6 spleen cells primed with syngenic B16 melanoma cells.(4) The initial findings show that radioisotope labeled monoclonal antibody is accumulated in melanoma bearing mice specifically and that these anti-bodies are distributed inhomogeneously in the tumors on macro-autoradiogram.(5) About 100 <mu> Ci of I-131 labeled antibody(M2590) and 30 <mu> Ci of I-125 labeled antibody(M562) were injected into the mice bearing syngenic mouse melanoma at the same time.(6) The distribution of I-131 labeled antibody was obtained on film within one week after the preparation of mouse specimen. About three months later the different distributions of the mouse antibody were observed on autoradiogram with the double nuclide technique.(7) This result suggests that preferential accumlations of two different antibody in the tumors were due to the antibodies directed against a determinant on tumor surface and that the polymonoclonal antibodies can be given to higher tumor uptakes and be useful for radioimmunodetection and radioimmunotherapy.

(1)放射性同位素标记单克隆抗体将用于播散性恶性肿瘤的成像和治疗。(2)本研究对小鼠黑色素瘤中抗黑色素瘤抗体（anti-melanoma antibody， AMAB）的不同分布进行了研究。(3) BALB/C骨髓瘤细胞与C57BL/6脾细胞融合，经同源性B16黑色素瘤细胞引发，获得单克隆抗体。(4)初步发现，放射性同位素标记的单克隆抗体在荷瘤小鼠体内特异性积累，宏观放射自显像显示，这些抗体在肿瘤内分布不均匀。(5)同时注射I-131标记抗体（M2590）的100 <mu> Ci和I-125标记抗体（M562）的30 <mu> Ci。(6)在小鼠标本制备后一周内获得I-131标记抗体在膜上的分布情况。约3个月后，用双核素自显影技术观察小鼠抗体的不同分布。(7)这一结果表明，两种不同的抗体在肿瘤中的优先积累是由于抗体针对肿瘤表面的一个决定因素，多单克隆抗体可以被给予更高的肿瘤摄取，并可用于放射免疫检测和放射免疫治疗。