Radioimmunoimaging of lung cancer with deglycosylated human monoclonal anti-lung

去糖基化人单克隆抗肺肿瘤的放射免疫成像

• 批准号: 06670940
• 负责人: KUSAKABE Kiyoko
• 金额: $ 1.41万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670940/
• 关键词: Radioimmunodetection; Radioimmunotherapy; human monoclonal anti-lung cancer antibody; HB4C5; ヒトモノクローナル抗体; 放射免疫検出法; 放射免疫治療法

The deglycosylated human monoclonal anti-lung cancer antibody, dHB4C5, which was obtained from lymph node metastasis in patient with lung cancer had been established by immunohistological and in vivo studies to be associated with lung cancer. As a preclinical study, this dHB4C5 was tried. to label with ^<111>In-DTPA which is adequate agent for radioimmunoimaging.^<111>In-DTPA labelled dHB4C5 showed higher accumulation to lung cancer xenografts in nude mice compared with ^<111>In-DTPA labeled control IgM.However, the remarkable uptake of ^<111>In-DTPA labelled compounds (dHB4C5, control human IgM) was observed in liver, kidney, and spleen. And, the low affinity of ^<111>In-DTPA to IgM was recognized.From these results including previous reports, ^<111>In-DTPA labelled IbM was thought to be limited application for clinical use.Then, we also evaluate the recombinated dHB4C5, C5-light chain (C5lambda chain), for the radioimmunodetection of lung ancer.

从肺癌患者的淋巴转移中获得脱糖基化的人抗肺癌单抗dHB4C5，经免疫组织化学和体内研究证实与肺癌相关。作为一项临床前研究，尝试了这种dHB4C5。~(111)In-DTPA标记的dHB4C5在裸鼠肺癌移植瘤中的蓄积量明显高于~(111)&gT；In-DTPA标记的对照IgM，但在肝、肾和脾中有明显的摄取。从已有的研究结果来看，标记IBM的重组HB4C5，C5轻链(C5lambda链)在临床上的应用受到限制。

项目成果

(社)日本アイソトープ協会医学薬学部会免役核医学専門委員会: "RI標識ヒトモノクローナル抗体の臨床応用に関する指針" RADIOISOTOPES. 44. 51-54 (1995)

日本同位素协会医学与药学科学委员会、免疫学与核医学专业委员会：《RI标记人单克隆抗体临床应用指南》RADIOISOTOPES 44. 51-54 (1995)。

日下部きよ子: "肺がんを認識するヒトモノクローナル抗肺がん抗体の臨床応用に関する基礎的検討" INNERVISION. 7. 37-38 (1994)

Kiyoko Kusakabe：“识别肺癌的人单克隆抗肺癌抗体的临床应用的基础研究”INNERVISION。 7. 37-38 (1994)。

日下部きよ子: "糖鎖を処理したヒトモノクローナル抗肺がん抗体,dHB4C5,による画像診断" INNERVISION. 8. 73- (1995)

Kiyoko Kusakabe：“使用聚糖处理的人单克隆抗肺癌抗体 dHB4C5 进行图像诊断”INNERVISION。 8. 73- (1995)

Kiyoko Kusakabe: "Accumulation Enhancement of Human Monoclonal Antibody ′HB4C5 to Lung Tumor Xenografts by N-Deglycosylation" Jounal of Nuclear Medicine. 35. 289-295 (1994)

Kiyoko Kusakabe：“通过 N-去糖基化增强人单克隆抗体 HB4C5 对肺肿瘤异种移植物的积累”核医学杂志 35. 289-295 (1994)

(社)日本アイソトープ協会医学 薬学部会免疫核医学専門委員会: "RI標識ヒトモノクローナル抗体の臨床応用に関する指針" RADIOISOTOPES. 44. 51-54 (1995)

日本同位素协会医学和药学科学核免疫学特别委员会：“RI标记的人单克隆抗体的临床应用指南”RADIOISOTOPES 44. 51-54 (1995)。