Radioimmunoimaging of lung cancer with deglycosylated human monoclonal anti-lung

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The deglycosylated human monoclonal anti-lung cancer antibody, dHB4C5, which was obtained from lymph node metastasis in patient with lung cancer had been established by immunohistological and in vivo studies to be associated with lung cancer. As a preclinical study, this dHB4C5 was tried. to label with ^<111>In-DTPA which is adequate agent for radioimmunoimaging.^<111>In-DTPA labelled dHB4C5 showed higher accumulation to lung cancer xenografts in nude mice compared with ^<111>In-DTPA labeled control IgM.However, the remarkable uptake of ^<111>In-DTPA labelled compounds (dHB4C5, control human IgM) was observed in liver, kidney, and spleen. And, the low affinity of ^<111>In-DTPA to IgM was recognized.From these results including previous reports, ^<111>In-DTPA labelled IbM was thought to be limited application for clinical use.Then, we also evaluate the recombinated dHB4C5, C5-light chain (C5lambda chain), for the radioimmunodetection of lung ancer.