The function of interleukin-2 and its receptor in B cell growth and differentiation

研究了活化的人B细胞上白细胞介素-2（IL-2）受体的特性及其在B细胞生长和分化中的作用。1. 正常人外周血B细胞在被SAC激活后开始表达IL-2受体。2. 经SDS-PAGE分析，活化B细胞上IL-2受体的表观分子量为60- 65kd。3. 它们被磷酸化了。4. B细胞表达高亲和力的IL-2受体。5. 正常活化的B细胞和活化的T细胞一样，表达两种不同的mRNA片段（1.5 kb和3.5 kb）。6. 正常活化的B细胞能够在外源性IL-2的作用下增殖。7. IL-2与干扰素共同诱导B细胞分化为免疫球蛋白分泌细胞。因此，我们的研究表明，至少在体外，IL-2能够促进B细胞的细胞生长和最终分化为产生免疫球蛋白的细胞。

The characteristics of the interleukin-2(IL-2) receptors on activated human B cells and their functions in B cell growth and differentiation have been studied. 1. Peripheral blood B cells from normal individuals become to express IL-2 receptors when activated by SAC. 2. IL-2 receptors on activated B cells have an apparent molecular weight of 60-65 KD by SDS-PAGE analysis. 3. They are phosphorylated. 4. B cells express high affinity IL-2 receptors. 5. Normal activated B cells, as activated T cells, express two different pieces(1.5 and 3.5 kb) of mRNA. 6. Normal activated B cells are able to proliferate in response to exogenous IL-2. 7. IL-2, toghether with interferon, induces B cell differentiation into immunoglobulin secreting cells. Thus, our studies have demonstrated that IL-2 is, at least in vitro, able to promote the cell growth and terminal differentiation into immunoglobulin producing cells in B cells.