Electroretinogram as a Monitor of Depth and Brain Ischemia, and as a model to study mechanisms of anesthesia.

视网膜电图作为深度和脑缺血的监测器，并作为研究麻醉机制的模型。

• 批准号: 60570720
• 负责人: TASHIRO Chikara
• 金额: $ 1.22万
• 依托单位: Dept. of Anesthesiology, Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570720/
• 关键词: volatile anesthetics; electroretinogram; (ERG); anesthetic depth; brain hypoxia; intraoperative monitoring

In the first experiment, the effects of volatile anesthetics, i.e. methoxyflurane, halothane, enflurane and isoflurane, on the electroretinogram (ERG) were studied in rabbits. The ERG was analyzed in terms of the a-wave and the first oscillatory component (OP-1) in the b-wave. The OP-1 peak latency showed a significant dose-related prolongation when anesthetic end-tidal concentrations were in excess of 0.8 MAC. The amplitudes of the a-wave and the O-1 decreased in dose-dependent manners, but their changes were less striking than those of the OP-1 latency. The peak latency of the a-wave remained unchanged.Using the same preparation, the effects of hypoxia were studied. After 3 min apnea, both peak latencies of the a-wave and the OP were prolonged and the amplitude of the a-wave decreased, while the anesthetics prolnged only the OPs' latencies. Hypoxic changes were observed under PaO2 less than 50 torr. Therefore, ERG would be a useful intraoperative monitoring to brain hypoxia and anesthetic depth.In the second experiment, anesthetic effects on the ERG were studied using a bull frog eye-cup preparation.Finnaly, we intended to develop the ERG monitoring system for anesthetic depth and brain hypoxia. For this purpose, we designed a special contact lens type ERG electrode equipped with a optical fiber which was connected to Xe strobophotic light sourse. In addition, a signal processing program was induced for rapid diagnosis. The clinical evaluation of the system is under the investigation.

实验一研究了吸入麻醉药甲氧氟醚、氟烷、安氟醚和异氟醚对家兔视网膜电图（ERG）的影响。根据a波和b波中的第一振荡分量（OP-1）分析ERG。当麻醉剂潮气末浓度超过0.8 MAC时，OP-1峰潜伏期显示出显著的剂量相关性延长。α波和O-1波幅呈剂量依赖性降低，但其变化不如OP-1潜伏期明显。用同样的方法，观察缺氧对脑组织a波潜伏期的影响。呼吸暂停3 min后，a波和OP的峰潜伏期均延长，a波振幅降低，而麻醉药仅延长OP的峰潜伏期。PaO_2 <50 ℃时，出现低通气性改变。实验二采用牛蛙眼杯模型研究麻醉对ERG的影响，并初步建立麻醉深度和脑缺氧的ERG监测系统。为此，我们设计了一种特殊的接触式透镜型ERG电极，它配有一根光纤，并与一个频闪光源相连。此外，还编制了信号处理程序，实现了快速诊断。该系统的临床评价正在调查中。

项目成果

Tashiro C, Muranishi R, Gomyo I, Mashimo T, Tomi K and Yoshiya I: "Electroretinogram as a possible monitor of anesthetic dept." Graefe's Arch Clin Exp Ophthalmol. 224. 473-476 (1986)

Tashiro C、Muranishi R、Gomyo I、Mashimo T、Tomi K 和 Yoshiya I：“视网膜电图作为麻醉科的可能监测器。”

Tashiro C;Muranishi R;Gomyo I;Mashimo T;Tomi K;Yoshiya I.: Grafe's Arch Clin Exp Ophthalmol. 224. 473-476 (1986)

Tashiro C;Muranishi R;Gomyo I;Mashimo T;Tomi K;Yoshiya I.：格拉夫拱门临床实验眼科。

Gomyo I, Muranishi R, Tashiro C, Uchida T and Yoshiya I: "The effects of enfourane and isoflurane on th electroretinogram in rabbits." Masui.

Gomyo I、Muranishi R、Tashiro C、Uchida T 和 Yoshiya I：“enfourane 和异氟烷对兔子视网膜电图的影响。”

後明郁男,村西隆一,太城力良,内田貴久,吉矢生人: 麻酔.

五名郁夫、村西龙一、大条力吉、内田隆久、伊木户吉也：《麻醉》。